Abstract | OBJECTIVE: METHODS:
Spinal cord ischemia was induced by means of balloon occlusion of the infrarenal aorta for 15 minutes at 39 degrees C in 18 New Zealand white rabbits. At 1, 3, and 7 days after reperfusion, 6 animals at each time point were killed, and the spinal cord was removed for histologic and immunohistochemical study. The variables analyzed were (1) neurologic function (Johnson score) at every 24 hours after reperfusion, (2) the number of intact motor neurons and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate- biotin nick-end labeling-positive positive neurons, and (3) expression of glial fibrillary acidic protein in the gray and white matter, which was expressed as the percentage of stained area. RESULTS: CONCLUSIONS: Reactive astrogliosis 3 days after transient spinal cord ischemia correlates with the number of intact motor neurons. Our method for semiquantitative analysis of reactive astrogliosis is simple and reproducible and seems useful for such experimental studies.
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Authors | Satoru Wakasa, Norihiko Shiiya, Tsuyoshi Tachibana, Tomonori Ooka, Yoshiro Matsui |
Journal | The Journal of thoracic and cardiovascular surgery
(J Thorac Cardiovasc Surg)
Vol. 137
Issue 4
Pg. 983-90
(Apr 2009)
ISSN: 1097-685X [Electronic] United States |
PMID | 19327528
(Publication Type: Journal Article)
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Topics |
- Animals
- Astrocytes
(pathology)
- Cell Death
- Disease Models, Animal
- Motor Neurons
(pathology)
- Rabbits
- Spinal Cord Ischemia
(pathology, physiopathology)
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