Icariin enhances neuronal survival after oxygen and glucose deprivation by increasing SIRT1.

Abstract

It has been reported that icariin protects neurons against ischemia/reperfusion injury. In this study, we found that icariin could enhance neuronal viability and suppress neuronal death after oxygen and glucose deprivation (OGD). Further study showed that neuroprotection by icariin was through the induction of Sirtuin type 1 (SIRT1), an effect that was reversed by SIRT1 inhibitor III and P38 inhibitor SB203580. SIRT1 is an endogenous gene of longevity, which increased neuronal viability and could be activated by stimulating the mitogen-activated protein kinase (MAPK) pathway. However, this study found that icariin activated the MAPK/P38 pathway, not the extracellular signal-regulated kinase (MAPK/ERK) or c-Jun N-terminal protein kinase (MAPK/JNK) to regulate SIRT1 expression. The results suggest that icariin may be developed into a neuroprotectant for ischemia-related brain injury.

• Authors: Lin Wang, Ling Zhang, Zhi-Bin Chen, Jia-Yong Wu, Xin Zhang, Yun Xu
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 609 Issue 1-3 Pg. 40-4 (May 01 2009) ISSN: 1879-0712 [Electronic] Netherlands
• PMID: 19303870 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Flavonoids
• Formazans
• Neuroprotective Agents
• Tetrazolium Salts
• MTT formazan
• L-Lactate Dehydrogenase
• Sirt1 protein, mouse
• Sirtuin 1
• Sirtuins
• Glucose
• Oxygen
• icariin

Topics

• Animals
• Cell Survival (drug effects)
• Cells, Cultured
• Cerebral Cortex (cytology)
• Embryo, Mammalian
• Flavonoids (pharmacology)
• Formazans (analysis, metabolism)
• Gene Expression Regulation, Enzymologic (drug effects)
• Glucose (pharmacology)
• L-Lactate Dehydrogenase (analysis, metabolism)
• Mice
• Neurons
• Neuroprotective Agents (pharmacology)
• Oxygen (pharmacology)
• Sirtuin 1
• Sirtuins (genetics, metabolism)
• Temperature
• Tetrazolium Salts (analysis, metabolism)
• Time Factors