The proteome of mesenteric lymph during acute pancreatitis and implications for treatment.

Abstract

CONTEXT:

The protein fraction of mesenteric lymph during acute pancreatitis and other critical illness is thought to contain toxic factors. However, we do not have a complete description of the mesenteric lymph proteome during acute pancreatitis.

OBJECTIVE:

The aim of this study was to define the proteomic changes in mesenteric lymph during acute pancreatitis.

SETTING:

Animal Laboratory, University of Auckland, New Zealand.

DESIGN:

Mesenteric lymph was collected from sixteen male Wistar rats randomised to Group 1 (n=8) with taurocholate induced acute pancreatitis and Group 2 (n=8) sham control. The lymph was subjected to proteomic analysis using iTRAQ (Applied Biosystems, Foster City, CA, USA) and liquid chromatography-tandem mass spectrometry.

RESULTS:

Two hundred and forty-five proteins including 35 hypothetical proteins were identified in mesenteric lymph. Eight of the 245 proteins had a significant increase in their relative abundance in acute pancreatitis conditioned mesenteric lymph, and 7 of these were pancreatic catabolic enzymes (pancreatic amylase 2, pancreatic lipase, carboxypeptidase A2, chymotrypsinogen B, carboxypeptidase B1, cationic trypsinogen, ribonuclease 1).

CONCLUSIONS:

This is the first comprehensive description of the proteome of mesenteric lymph during acute pancreatitis and has demonstrated a significantly increased relative abundance of 7 secreted pancreatic catabolic enzymes in acute pancreatitis conditioned mesenteric lymph. This study provides a clear rationale for further research to investigate the efficacy of enteral protease inhibitors in the treatment of acute pancreatitis.

• Authors: Anubhav Mittal, Anthony R J Phillips, Martin Middleditch, Katya Ruggiero, Benjamin Loveday, Brett Delahunt, Garth J S Cooper, John A Windsor
• Journal: JOP : Journal of the pancreas (JOP) Vol. 10 Issue 2 Pg. 130-42 (Mar 09 2009) ISSN: 1590-8577 [Electronic] Italy
• PMID: 19287105 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Proteome
• Taurocholic Acid

Topics

• Acute Disease
• Animals
• Chromatography, Liquid (methods)
• Databases, Protein
• Lymph (metabolism)
• Male
• Mesentery
• Pancreatitis (chemically induced, metabolism, therapy)
• Proteome (analysis)
• Proteomics (methods)
• Random Allocation
• Rats
• Rats, Wistar
• Reproducibility of Results
• Spectrometry, Mass, Electrospray Ionization (methods)
• Taurocholic Acid