Abstract | BACKGROUND & AIMS: Despite the proven ability of immunization to reduce Helicobacter infection in mouse models, the precise mechanism of protection has remained elusive. This study explores the possibility that interleukin (IL)-17 plays a role in the reduction of Helicobacter infection following vaccination of wild-type animals or in spontaneous reduction of bacterial infection in IL-10-deficient mice. METHODS: RESULTS: Gastric IL-17 levels were strongly increased in mice mucosally immunized with urease plus cholera toxin and challenged with Helicobacter felis as compared with controls (654 +/- 455 and 34 +/- 84 relative units for IL-17 messenger RNA expression [P < .01] and 6.9 +/- 8.4 and 0.02 +/- 0.04 pg for IL-17 protein concentration [P < .01], respectively). Flow cytometry analysis showed that a peak of CD4(+)IL-17(+) T cells infiltrating the gastric mucosa occurred in immunized mice in contrast to control mice (4.7% +/- 0.3% and 1.4% +/- 0.3% [P < .01], respectively). Gastric mucosa-infiltrating CD4(+)IL-17(+) T cells were also observed in IL-10-deficient mice that spontaneously reduced H felis infection (4.3% +/- 2.3% and 2% +/- 0.6% [P < .01], for infected and noninfected IL-10-deficient mice, respectively). In wild-type immunized mice, intraperitoneal injection of anti-IL-17 antibodies significantly inhibited inflammation and the reduction of Helicobacter infection in comparison with control antibodies (1 of 12 mice vs 9 of 12 mice reduced Helicobacter infection [P < .01], respectively). CONCLUSIONS:
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Authors | Dominique Velin, Laurent Favre, Eric Bernasconi, Daniel Bachmann, Catherine Pythoud, Essia Saiji, Hanifa Bouzourene, Pierre Michetti |
Journal | Gastroenterology
(Gastroenterology)
Vol. 136
Issue 7
Pg. 2237-2246.e1
(Jun 2009)
ISSN: 1528-0012 [Electronic] United States |
PMID | 19272385
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Bacterial Vaccines
- Cytokines
- Interleukin-17
- Interleukin-10
- Peroxidase
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Topics |
- Animals
- Bacterial Vaccines
(pharmacology)
- Cytokines
(metabolism)
- Disease Models, Animal
- Female
- Flow Cytometry
- Gastric Mucosa
(immunology, metabolism, pathology)
- Helicobacter Infections
(immunology, metabolism, prevention & control)
- Helicobacter pylori
(immunology)
- Immunohistochemistry
- Immunologic Memory
(physiology)
- Interleukin-10
(deficiency, immunology)
- Interleukin-17
(immunology, metabolism)
- Mice
- Mice, Inbred C57BL
- Peroxidase
(metabolism)
- Polymerase Chain Reaction
- Probability
- Random Allocation
- Sensitivity and Specificity
- Statistics, Nonparametric
- Th1 Cells
(immunology)
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