We investigated the effects of Heterodera glycines
cyst components and surface disinfestants on hatching of H. glycines eggs in vitro. Eggs were incubated in either H. glycines
cyst wall fragments,
cyst wall and egg rinsate, egg homogenate, or control solutions of soybean root diffusate, sterile distilled water, or
zinc sulfate. Hatch in
cyst wall and egg rinsate, and egg homogenate, was greater (alpha = 0.05) than hatch in sterile distilled water; however, it was not different from hatch in
zinc sulfate according to Dunnett's test. Hatch in
cyst wall fragments was similar to hatch in sterile distilled water. To determine whether surface disinfestants affected hatch, eggs were treated first with
chlorhexidine diacetate,
mercuric chloride,
sodium hypochlorite, or
streptomycin sulfate and then incubated in H. glycines egg homogenate, soybean root diffusate, sterile distilled water, or
zinc sulfate. Hatch of eggs treated with
chlorhexidine diacetate,
mercuric chloride, and
streptomycin sulfate was reduced (alpha = 0.05), and hatch of eggs treated with
sodium hypochlorite was increased (alpha = 0.05) relative to hatch of nontreated eggs in all incubation solutions except
zinc sulfate according to Dunnett's Test. Hatch in
zinc sulfate was similar among all surface disinfestants except
mercuric chloride, where hatch was reduced relative to hatch of nontreated and other surface disinfestant-treated eggs.