Abstract |
Several antigens derived from hepatitis E virus (HEV) genotype 1 strains have shown immunogenicity and efficacy against hepatitis E in primates and humans. However, the protective effect of a vaccine derived from HEV genotype 4 has not been studied. This study aimed to evaluate the immunogenicity and protective efficacy of the T1-ORF2 (56 kDa) capsid protein derived from HEV strain T1 (genotype 4) in rhesus monkeys. Two doses (40 microg) of alum-absorbed T1-ORF2 capsid protein were administered 4 weeks apart. Seroconversion occurred in all immunized monkeys 1-2 weeks after the first dose. The peak levels of anti-HEV IgG appeared at 2-3 weeks after the second dose and ranged from 5.7 to 196.0 U/ml. All monkeys showed an anamnestic antibody response to the second dose. Control monkeys immunized with saline remained negative for HEV antibodies throughout the pre-challenge period. The immunized monkeys were challenged intravenously with HEV genotypes 1 and 4. Monkeys immunized with T1-ORF2 were protected from infection and hepatitis after challenge with 5 x 10(4) genome equivalents of HEV, regardless of the genotype. After challenge with 5 x 10(5) genome equivalents of HEV genotype 4, the monkeys immunized with T1-ORF2 had a shorter period of raised alanine aminotransferase levels and a shorter duration of fecal shedding compared to control monkeys immunized with saline. In conclusion, these results suggest that, in rhesus monkeys, the T1-ORF2 capsid protein of HEV genotype 4 has similar cross-protective effects to other candidate vaccines derived from HEV genotype 1.
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Authors | W J Huang, H Y Zhang, T J Harrison, H Y Lan, G Y Huang, Y C Wang |
Journal | Archives of virology
(Arch Virol)
Vol. 154
Issue 3
Pg. 481-8
( 2009)
ISSN: 1432-8798 [Electronic] Austria |
PMID | 19240977
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adjuvants, Immunologic
- Alum Compounds
- Antibodies, Viral
- Immunoglobulin G
- ORF2 protein, Hepatitis E virus
- Vaccines, Synthetic
- Viral Hepatitis Vaccines
- Viral Proteins
- aluminum sulfate
- Alanine Transaminase
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Topics |
- Adjuvants, Immunologic
(pharmacology)
- Alanine Transaminase
(blood)
- Alum Compounds
(pharmacology)
- Animals
- Antibodies, Viral
(blood)
- Feces
(virology)
- Hepatitis E
(prevention & control)
- Hepatitis E virus
(genetics, immunology)
- Immunization, Secondary
- Immunoglobulin G
(blood)
- Macaca mulatta
- Vaccines, Synthetic
(genetics, immunology)
- Viral Hepatitis Vaccines
(genetics, immunology)
- Viral Proteins
(genetics, immunology)
- Virus Shedding
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