Successful establishment of MEKC with electrochemiluminescence detection based on one functionalized ionic liquid.

Abstract

Herein, one water-soluble functionalized ionic liquid, 1-butyl-3-methylimidazolium dodecyl sulfate ([BMIm(+)][C(12)H(25)SO(-) (4)]), was designed and its superiorities either used as supporting electrolytes or as additives for successful establishment of MEKC with electrochemiluminescence (ECL) detection (MEKC-ECL) method were investigated. Compared with the common supporting electrolytes such as phosphate solution, 1-butyl-3-methylimidazolium dodecyl sulfate solution used as running buffers led to greatly enhanced ECL intensities and column efficiencies for negative targets, a little increase for neutral-charge ones while maintained nearly unchanged for positive ones due to the electrostatic forces between the large cation BMIm(+) and the solutes and the hydrophobic interactions resulting from the large anion C(12)H(25)SO(-) (4). Moreover, resolution efficiency between analytes was significantly improved. As the existence of ionic liquid moiety, BMIm(+), accelerated the electron transfer at the electrode surface, the poisoning effect of long chain C(12)H(25)SO(-) (4) on the electrode surface was eliminated and reproducible ECL intensities with relative standard derivation of 1.02% (n=6) were obtained. The proposed novel MEKC-ECL system was again validated by the baseline separated two similar amino acids of proline and hydroxyproline with lower detection limits of 0.5 and 0.8 microM (S/N=3), respectively.

• Authors: Yuanhong Xu, Lanyun Fang, Erkang Wang
• Journal: Electrophoresis (Electrophoresis) Vol. 30 Issue 2 Pg. 365-71 (Jan 2009) ISSN: 1522-2683 [Electronic] Germany
• PMID: 19204950 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Validation Study)

Chemical References

• 1-butyl-3-methylimidazolium
• Imidazoles
• Ionic Liquids
• Water
• Proline
• Hydroxyproline

Topics

• Chromatography, Micellar Electrokinetic Capillary (methods)
• Electrochemistry (methods)
• Hydroxyproline (chemistry)
• Imidazoles (chemistry)
• Ionic Liquids (chemistry)
• Luminescent Measurements (methods)
• Proline (chemistry)
• Sensitivity and Specificity
• Solubility
• Water (chemistry)