In vivo imaging of the mouse model of X-linked juvenile retinoschisis with fourier domain optical coherence tomography.

Abstract

PURPOSE:

The purpose of this study was to investigate Fourier domain optical coherence tomography (FD OCT) as a noninvasive tool for retinal imaging in the Rs1h-knockout mouse (model for X-linked juvenile retinoschisis).

METHODS:

A prototype spectrometer-based FD OCT system was used in combination with a custom optical beam-scanning platform. Images of the retinas from wild-type and Rs1h-knockout mice were acquired noninvasively with FD OCT with the specimen anesthetized. At the completion of the noninvasive FD OCT imaging, invasive retinal cross-sectional images (histology) were acquired from a nearby region for comparison to the FD OCT images.

RESULTS:

The retinal layers were identifiable in the FD OCT images, permitting delineation and thickness measurement of the outer nuclear layer (ONL). During FD OCT in vivo imaging of the Rs1h-knockout mouse, holes were observed in the inner nuclear layer (INL), and retinal cell disorganization was observed as a change in the backscattering intensity profile. Comparison of the ONL measurements acquired noninvasively with FD OCT to measurements taken using histology at nearby locations showed a degeneration of roughly 30% of the ONL by the age of 2 months in Rs1h-knockout mice relative to wild-type.

CONCLUSIONS:

FD OCT was demonstrated to be effective for noninvasive imaging of retinal degeneration and observation of retinal holes in Rs1h-knockout mice.

• Authors: Jing Xu, Laurie L Molday, Robert S Molday, Marinko V Sarunic
• Journal: Investigative ophthalmology & visual science (Invest Ophthalmol Vis Sci) Vol. 50 Issue 6 Pg. 2989-93 (Jun 2009) ISSN: 1552-5783 [Electronic] United States
• PMID: 19182246 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Cell Adhesion Molecules
• Eye Proteins
• RS1 protein, mouse

Topics

• Animals
• Body Weights and Measures
• Cell Adhesion Molecules (genetics)
• Diagnostic Techniques, Ophthalmological
• Disease Models, Animal
• Eye Proteins (genetics)
• Fourier Analysis
• Mice
• Mice, Knockout
• Microscopy, Fluorescence
• Retinal Neurons (pathology)
• Retinoschisis (genetics, pathology)
• Tomography, Optical Coherence