Abstract | BACKGROUND: Inhibitory effects of roflumilast on responses characteristic of allergic asthma were investigated in a fungal asthma model in BALB/c mice. METHODS: Mice were sensitized with Aspergillus antigen (Afu) and exposed to Afu or vehicle, and given roflumilast 1 or 5 mg/kg. Early airway response (EAR) and late airway hyperresponsiveness (AHR) to methacholine were measured via plethysmography. Bronchoalveolar lavage (BAL) was used to assess inflammatory cell count. RESULTS: In Afu-exposed mice, roflumilast dose-dependently reduced the EAR [26% at 1 mg/kg (NS) and 94% at 5 mg/kg (p < 0.01)] and AHR [46% at 1 mg/kg (NS) and 128% at 5 mg/kg (p < 0.05)]. Roflumilast 5 mg/kg reduced neutrophil, eosinophil and lymphocyte counts [87% (p < 0.01), 40% (NS) and 67% (p < 0.01), respectively] in BAL fluid versus controls. CONCLUSIONS: In this model, roflumilast inhibited the EAR, suppressed AHR and reduced inflammatory cell infiltration.
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Authors | Heinz-Gerd Hoymann, Lutz Wollin, Meike Muller, Regina Korolewitz, Norbert Krug, Armin Braun, Rolf Beume |
Journal | Pharmacology
(Pharmacology)
Vol. 83
Issue 3
Pg. 188-95
( 2009)
ISSN: 1423-0313 [Electronic] Switzerland |
PMID | 19176983
(Publication Type: Journal Article)
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Copyright | 2009 S. Karger AG, Basel. |
Chemical References |
- Aminopyridines
- Antigens, Fungal
- Benzamides
- Cyclopropanes
- Phosphodiesterase 4 Inhibitors
- Roflumilast
- Methacholine Chloride
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Topics |
- Aminopyridines
(pharmacology)
- Animals
- Antigens, Fungal
(immunology)
- Aspergillus fumigatus
(immunology)
- Asthma
(drug therapy, metabolism, physiopathology)
- Benzamides
(pharmacology)
- Bronchial Hyperreactivity
(drug therapy)
- Bronchoalveolar Lavage Fluid
(cytology)
- Cyclopropanes
(pharmacology)
- Disease Models, Animal
- Eosinophils
(cytology)
- Female
- Leukocyte Count
- Lymphocytes
(cytology)
- Methacholine Chloride
(pharmacology)
- Mice
- Mice, Inbred BALB C
- Neutrophils
(cytology)
- Phosphodiesterase 4 Inhibitors
- Pneumonia
(metabolism)
- Respiratory Hypersensitivity
(drug therapy)
- Respiratory Mechanics
(drug effects)
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