[Expression of PML-RAR alpha fusion transcripts detection by using RQ-PCR].

Abstract

OBJECTIVE:

To establish the real time quantitative PCR (RQ-PCR) assay according to 'Europe Against Cancer' (EAC) program and analyze the results of detection and quantification of different PML-RAR alpha transcript isoforms in patients with acute promyelocytic leukemia (APL).

METHODS:

Three RQ-PCR systems were performed to detect the most frequent PML-RAR alpha transcripts (L-form, S-form and V-form) in 30 APL patients and the RQ-PCR end-point products were identified by electrophoresis.

RESULTS:

S-form RQ-PCR system could amplify positive signals of three isoforms in all of 30 cases, and V-form RQ-PCR system could do so in both L-form and V-form positive cases, however, L-form RQ-PCR system could only do so in L-form-positive cases. Electrophoresis and sequencing of end-point products amplified by S-form RQ-PCR system revealed three bands in each of L-form (621 bp, 477 bp and 218 bp) and V-form (567 bp, 423 bp and 218 bp) positive patients samples.

CONCLUSIONS:

RQ-PCR, sensitive and reliable, can be used for monitoring the minimal residual disease in APL patients, however, its results should be interpreted carefully if it is used for detection of PML-RAR alpha fusion transcripts prospectively.

• Authors: Lan-xiu Han, Jiang Lin, Jun Qian, Ya-li Wang, Zhen Qian, Xiao-fei Yang, Xiao-jing Sheng, Dong-ming Yao
• Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi (Zhonghua Xue Ye Xue Za Zhi) Vol. 29 Issue 11 Pg. 753-6 (Nov 2008) ISSN: 0253-2727 [Print] China
• PMID: 19176013 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Oncogene Proteins, Fusion
• promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein

Topics

• Adolescent
• Adult
• Child
• Female
• Humans
• Leukemia, Promyelocytic, Acute (diagnosis, genetics)
• Male
• Middle Aged
• Neoplasm, Residual (diagnosis, genetics)
• Oncogene Proteins, Fusion (genetics)
• Reverse Transcriptase Polymerase Chain Reaction (methods)
• Sensitivity and Specificity
• Young Adult