Abstract |
The erythrocyte glutathione reductase activation coefficient (EGRAC) is an index of riboflavin deficiency or, more exactly, of FAD deficiency in man. In this report a sensitive version of the EGRAC test is introduced that is based on the molecular properties of glutathione reductase and its FAD-free apoenzyme. The hemoglobin concentration of the blood sample can be estimated simultaneously using the spectrophotometric absorption at 340 nm. - The method was tested for 33 thyroidectomized patients in comparison with a euthyroid control group. From the average EGRAC values (1.40 vs. 1.22) it was deduced that the average free FAD level was approximately 2 times lower for the patients' than for the control group. Discussed is the role of the EGRAC test in hormonal and nutritional disorders.
|
Authors | K Becker, B Krebs, R H Schirmer |
Journal | International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition
(Int J Vitam Nutr Res)
Vol. 61
Issue 2
Pg. 180-7
( 1991)
ISSN: 0300-9831 [Print] Switzerland |
PMID | 1917356
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
|
Topics |
- Adult
- Aged
- Aged, 80 and over
- Enzyme Activation
- Erythrocytes
(enzymology)
- Glutathione Reductase
(blood, standards)
- Humans
- Hypothyroidism
(blood, diagnosis)
- Middle Aged
- Reference Standards
- Reference Values
- Reproducibility of Results
- Riboflavin Deficiency
(blood, diagnosis)
- Thyroidectomy
|