Hydroxyethyl starch 130/0.4 prevents the early pulmonary inflammatory response and oxidative stress after hemorrhagic shock and resuscitation in rats.

Abstract

BACKGROUND:

This study was designed to determine the effects of various resuscitation fluids on pulmonary capillary leakage and pulmonary edema after HS and fluid resuscitation (HS/R) and to determine whether an antiinflammatory or antioxidative mechanism was involved.

METHODS:

We induced HS by bleeding male Sprague-Dawley rats to a blood pressure of 30 to 40 mm Hg for 60 min. 60 min later, the rats were killed (HS group) or immediately resuscitated with L-isomer lactated Ringer's solution (HS+LR group), shed blood (HS+BL group), or hydroxyethyl starch (HS+HES group) to maintain the blood pressure to the original value during the 60-min resuscitation period. 3 h after resuscitation, pulmonary capillary leakage and wet/dry weight ratio, levels of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, malondialdehyde (MDA), oxidized and reduced glutathione (GSH and GSSG), myeloperoxidase (MPO) activity, nuclear factor (NF)-kappaB, activator protein (AP)-1 activation, and lung microscopic and ultrastructural histological changes were measured.

RESULTS:

HES and BL treatment significantly improved pulmonary capillary leakage, wet/dry weight ratio and lung injuries after HS/R. In addition, both HES and BL could attenuate the increase in TNF-alpha, IL-6, MPO levels and NF-kappaB activation. However, HES but not BL could attenuate the increase in MDA level and GSSH/GSH ratio and AP-1 activation.

CONCLUSIONS:

HES might attenuate pulmonary injuries by modulating pulmonary inflammatory response and oxidative stress, whereas BL attenuates pulmonary injuries by modulating pulmonary inflammatory response but not oxidative stress.

• Authors: Pengfei Wang, Yousheng Li, Jieshou Li
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 9 Issue 3 Pg. 347-53 (Mar 2009) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 19166983 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hydroxyethyl Starch Derivatives
• Interleukin-6
• NF-kappa B
• Plasma Substitutes
• Transcription Factor AP-1
• Tumor Necrosis Factor-alpha
• Malondialdehyde
• Peroxidase
• Glutathione
• Glutathione Disulfide

Topics

• Animals
• Blood Pressure (drug effects, physiology)
• Capillary Permeability (drug effects)
• Disease Models, Animal
• Glutathione (metabolism)
• Glutathione Disulfide (metabolism)
• Hydroxyethyl Starch Derivatives (therapeutic use)
• Interleukin-6 (metabolism)
• Male
• Malondialdehyde (metabolism)
• NF-kappa B (metabolism)
• Oxidative Stress (drug effects)
• Peroxidase (metabolism)
• Plasma Substitutes (pharmacology)
• Pneumonia (etiology, prevention & control)
• Pulmonary Circulation (drug effects)
• Rats
• Rats, Sprague-Dawley
• Resuscitation
• Shock, Hemorrhagic (complications)
• Transcription Factor AP-1 (metabolism)
• Tumor Necrosis Factor-alpha (metabolism)