Abstract | BACKGROUND: Helicobacter pylori infection is associated with development of chronic inflammation and infiltration of immune cells into the gastric mucosa. As unconventional T-lymphocytes expressing natural killer cell receptors are considered to play central roles in the immune response against infection, a study investigating their frequencies in normal and H. pylori-infected gastric mucosa was undertaken. MATERIALS AND METHODS: Flow cytometry was used to quantify T-cells expressing the natural killer cell markers CD161, CD56, and CD94 in freshly isolated lymphocytes from the epithelial and lamina propria layers of gastric mucosa. Thirteen H. pylori-positive and 24 H. pylori-negative individuals were studied. RESULTS: CD94(+) T-cells were the most abundant (up to 40%) natural killer receptor-positive T-cell population in epithelial and lamina propria layers of H. pylori-negative gastric mucosa. CD161(+) T-cells accounted for about one-third of all T-cells in both compartments, but the lowest proportion were of CD56(+) T-cells. Compared with H. pylori-negative mucosa, in H. pylori-infected mucosa the numbers of CD161(+) T-cells were significantly greater (p = .04) in the epithelium, whereas the numbers of CD56(+) T-cells were lower (p = .01) in the lamina propria. A minor population (< 2%) of T-cells in both mucosal layers of H. pylori-negative subjects were natural killer T-cells, and whose proportions were not significantly different (p > .05) to those in H. pylori-infected individuals. CONCLUSIONS: The predominance, heterogeneity, and distribution of natural killer cell receptor-positive T-cells at different locations within the gastric mucosa reflects a potential functional role during H. pylori infection and warrants further investigation.
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Authors | Joan O'Keeffe, Carol M Gately, Yvonne O'Donoghue, Syed A Zulquernain, Fiona M Stevens, Anthony P Moran |
Journal | Helicobacter
(Helicobacter)
Vol. 13
Issue 6
Pg. 500-5
(Dec 2008)
ISSN: 1523-5378 [Electronic] England |
PMID | 19166415
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CD56 Antigen
- NK Cell Lectin-Like Receptor Subfamily B
- NK Cell Lectin-Like Receptor Subfamily D
- Receptors, Natural Killer Cell
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Topics |
- Adolescent
- Adult
- Aged
- CD56 Antigen
(analysis)
- Female
- Flow Cytometry
- Gastric Mucosa
(immunology)
- Helicobacter Infections
(immunology)
- Helicobacter pylori
(immunology)
- Humans
- Male
- Middle Aged
- NK Cell Lectin-Like Receptor Subfamily B
(analysis)
- NK Cell Lectin-Like Receptor Subfamily D
(analysis)
- Receptors, Natural Killer Cell
(biosynthesis)
- T-Lymphocyte Subsets
(chemistry, immunology)
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