Autoradiographic localization of prolactin receptors in 7,12-dimethylbnez[a]anthracene-induced rat mammary carcinoma.

Abstract

Prolactin receptors were localized by autoradiography in 7,12-dimethylbenz[a]anthracene (DMBA)-induced mammary tumors from rats by incubation of tumor slices with [125I]ovine prolactin. Specific prolactin binding was confined to tumor cells, and nonspecific binding was present in alveolar spaces and connective tissue. In some tumors, all cells contained receptors; in others, up to one-half the cells remained unlabeled. These results suggested that variation in receptor content in DMBA-induced mammary tumors may be caused by two distinct populations of cells--one which contains receptors and another which possesses very few receptor sites or none at all.

• Authors: M E Costlow, W L McGuire
• Journal: Journal of the National Cancer Institute (J Natl Cancer Inst) Vol. 58 Issue 4 Pg. 1173-5 (Apr 1977) ISSN: 0027-8874 [Print] United States
• PMID: 191627 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Benz(a)Anthracenes
• Receptors, Cell Surface
• 9,10-Dimethyl-1,2-benzanthracene
• Prolactin

Topics

• 9,10-Dimethyl-1,2-benzanthracene
• Animals
• Autoradiography
• Benz(a)Anthracenes
• Female
• Mammary Neoplasms, Experimental (chemically induced, metabolism)
• Prolactin (metabolism)
• Rats
• Receptors, Cell Surface