Generators of reactive oxygen forms gamma-irradiation and ascorbic acid--cobalt metallocomplexes induced large-scale fragmentation and reparation of DNA in tumor cells.

Abstract

It was demonstrated that ascorbate-cobalt phthalocyanine complex produces a time-dependent nuclease effect on leukemia K-562 cells is. Catalase added to the incubation medium prevented or blocked fragmentation of cell DNA. The size of large-scale fragments formed during irradiation and exposure to the above system varied from 2200 to 30 kbp. The fragments induced by the system recombined slower than the fragments induced by g-irradiation in a dose adequate by the level of DNA damage. This effect observed previously in HEp-2 carcinoma cells exposed to the action of the B12b+C vitamin system can be explained by generation of H(2)O(2) inducing more severe damage to DNA structure than gamma-radiation due to site-specific Fenton reaction.

• Authors: A I Medvedev, V V Leschenko
• Journal: Bulletin of experimental biology and medicine (Bull Exp Biol Med) Vol. 145 Issue 5 Pg. 600-3 (May 2008) ISSN: 0007-4888 [Print] United States
• PMID: 19145291 (Publication Type: Journal Article)

Chemical References

• DNA, Neoplasm
• Indoles
• Organometallic Compounds
• Reactive Oxygen Species
• cobalt phthalocyanine
• Ascorbic Acid

Topics

• Apoptosis (drug effects, radiation effects)
• Ascorbic Acid (pharmacology)
• DNA Fragmentation (drug effects, radiation effects)
• DNA Repair (drug effects, radiation effects)
• DNA, Neoplasm (drug effects, metabolism, radiation effects)
• Gamma Rays
• Humans
• Indoles (pharmacology)
• K562 Cells
• Organometallic Compounds (pharmacology)
• Reactive Oxygen Species (metabolism, radiation effects)