HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Pathogenesis of Mycobacterium avium subsp. paratuberculosis in neonatal calves after oral or intraperitoneal experimental infection.

Abstract
Understanding the host response to Mycobacterium avium subsp. paratuberculosis is critical to the development of effective vaccines and therapeutics for the control of this disease in the field. The current study compared the effectiveness of oral and intraperitoneal (IP) methods of experimental inoculation and two strains of M. avium subsp. paratuberculosis (strain K-10 and clinical isolate 509) on the level of infection and lesion development. Calves were inoculated with 4x10(11) to 8x10(12)cfu live bacteria, depending upon treatment group. Fecal shedding of M. avium subsp. paratuberculosis was minimal and infrequent over the course of the study for calves that received strain K-10 (oral and IP), however, calves orally inoculated with the clinical isolate shed high numbers of bacteria in their feces up to 4 months post-inoculation. Colonization was present in a number of intestinal tissues and lymph nodes with the lowest number of affected tissues in the IP calves and the highest for calves receiving the clinical isolate via oral inoculation. Microscopic lesions were predominantly found in the ileal and jejunal sections of small intestine and their associated lymph nodes, as well as the ileocecal valve and node. These data suggest that a variety of experimental infection regimes can be effective but oral inoculation with a clinical isolate may result in greater colonization of tissues and fecal shedding of M. avium subsp. paratuberculosis.
AuthorsJ R Stabel, M V Palmer, B Harris, B Plattner, J Hostetter, S Robbe-Austerman
JournalVeterinary microbiology (Vet Microbiol) Vol. 136 Issue 3-4 Pg. 306-13 (May 12 2009) ISSN: 0378-1135 [Print] Netherlands
PMID19135813 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References
  • DNA, Bacterial
  • Dexamethasone
Topics
  • Animals
  • Animals, Newborn
  • Cattle
  • Cattle Diseases (microbiology)
  • Colony Count, Microbial
  • DNA, Bacterial (chemistry, genetics)
  • Dexamethasone (pharmacology)
  • Disease Models, Animal
  • Feces (microbiology)
  • Female
  • Genotype
  • Histocytochemistry (veterinary)
  • Host-Pathogen Interactions
  • Intestinal Diseases (microbiology, veterinary)
  • Mycobacterium avium subsp. paratuberculosis (pathogenicity, physiology)
  • Paratuberculosis (microbiology)
  • Polymerase Chain Reaction (veterinary)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: