Abstract | PURPOSE: METHODS:
Glyceraldehyde was derivatized to a fluorescent compound, and the compound was then quantified by high-performance liquid chromatography. RESULTS: The lens glyceraldehyde levels in normal and diabetic rats were 0.75 +/- 0.06 and 1.26 +/- 0.21 nmol/g wet weight (means +/- standard deviations of 6 animals, p < 0.01), respectively. Isolated rat lenses accumulated a higher level of glyceraldehyde when cultured for 6 days in 25.5 mM glucose than when cultured in 5.5 mM glucose. CONCLUSIONS:
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Authors | I Miwa, A-S Chen, T Taguchi |
Journal | Ophthalmic research
(Ophthalmic Res)
Vol. 41
Issue 2
Pg. 98-101
( 2009)
ISSN: 1423-0259 [Electronic] Switzerland |
PMID | 19122471
(Publication Type: Journal Article)
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Copyright | (c) 2008 S. Karger AG, Basel. |
Chemical References |
- 2-amino-6-(3-oxidopyridinium-1-yl)hexanoic acid
- Benzofurans
- Blood Glucose
- Hydantoins
- Pyridinium Compounds
- M 16287
- Glyceraldehyde
- Aldehyde Reductase
- Lysine
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Topics |
- Aldehyde Reductase
(antagonists & inhibitors)
- Animals
- Benzofurans
(pharmacology)
- Blood Glucose
(analysis)
- Cataract
(metabolism)
- Chromatography, High Pressure Liquid
- Diabetes Mellitus, Type 2
(metabolism)
- Disease Models, Animal
- Glyceraldehyde
(metabolism)
- Hydantoins
(pharmacology)
- Lens, Crystalline
(drug effects, metabolism)
- Lysine
(analogs & derivatives, metabolism)
- Male
- Pyridinium Compounds
(metabolism)
- Rats
- Rats, Wistar
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