Abstract | OBJECT: The origin of spinal congenital dermal sinuses is not known. A local nondisjunction of the closing neural tube and the epidermal ectoderm is thought to be the cause of this malformation. In this experimental study, a nondisjunction was mimicked in chick embryos to create an animal model for the dermal sinus. METHODS: A piece of amniotic tissue was implanted in the closing neural tube in ovo in chick embryos at 2 days of incubation. A total of 50 embryos were manipulated. After a further incubation time of 2-7 days, the embryos were macroscopically and histologically evaluated. RESULTS: Dermal sinus-like anomalies were induced in 24 embryos. The induced abnormalities varied from superficial, epidermal lesions to epidermal dimples continuing as a strand of tissue toward the neural tube. This strand invariably was of nonneuronal origin. Additionally, in 3 embryos a split cord malformation was noted, most likely caused by damage to the neural tube during implantation. CONCLUSIONS: Implantation of donor amniotic tissue in the closing chick neural tube does result in a dimple, from which a strand of tissue continues to the neural tube in various cases, indicating that formation of a dermal sinus-like anomaly can be successfully induced by experimental continuation of the connection between neural tube and surface ectoderm. This finding strengthens the hypothesis that a human dermal sinus arises after nondisjunction of neural tube and surface ectoderm.
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Authors | Jasper van Aalst, Toon F M Boselie, Emile A M Beuls, Johan S H Vles, Henny W M van Straaten |
Journal | Journal of neurosurgery. Pediatrics
(J Neurosurg Pediatr)
Vol. 3
Issue 1
Pg. 24-8
(Jan 2009)
ISSN: 1933-0707 [Print] United States |
PMID | 19119900
(Publication Type: Journal Article)
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Topics |
- Animals
- Chick Embryo
- Disease Models, Animal
- Ectoderm
(pathology)
- Neural Tube
(embryology, pathology)
- Neural Tube Defects
(embryology, pathology)
- Notochord
(embryology, pathology)
- Spina Bifida Occulta
(embryology, pathology)
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