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Antiproliferative activity and apoptosis-inducing mechanism of Concanavalin A on human melanoma A375 cells.

Abstract
The objective of this study was to investigate the antiproliferative activity and apoptosis-inducing mechanism of Concanavalin A (ConA) on human melanoma A375 cells. We firstly simulated the three-dimensional structure of ConA. Subsequently, we found that ConA possessed remarkable antiproliferative effect on A375 cells. Further experimental data indicated that there was a link between its hemagglutinating activity, mannose-binding activity and antiproliferative activity. In addition, we showed that ConA induced A375 cell apoptosis in a caspase-dependent manner. Then, we demonstrated that the treatment of ConA caused mitochondrial transmembrane potential (MMP) collapse, cytochrome c release and caspase activation. In conclusion, we report for the first time that there may be a close correlation between carbohydrate-binding activity of ConA and its antiproliferative activity. Also, we demonstrate firstly that ConA induces A375 cell death in a caspase-dependent manner as well as through a mitochondrial apoptotic pathway.
AuthorsBo Liu, Chun-yang Li, He-jiao Bian, Ming-wei Min, Long-fei Chen, Jin-ku Bao
JournalArchives of biochemistry and biophysics (Arch Biochem Biophys) Vol. 482 Issue 1-2 Pg. 1-6 (Feb 2009) ISSN: 1096-0384 [Electronic] United States
PMID19111670 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Concanavalin A
  • Caspase 3
  • Caspase 8
  • Caspase 9
  • Mannose
Topics
  • Apoptosis (drug effects)
  • Caspase 3 (metabolism)
  • Caspase 8 (metabolism)
  • Caspase 9 (metabolism)
  • Cell Division (drug effects)
  • Cell Line, Tumor
  • Computer Simulation
  • Concanavalin A (chemistry, pharmacology)
  • Hemagglutination (drug effects)
  • Humans
  • Mannose (pharmacology)
  • Melanoma
  • Membrane Potentials (drug effects)
  • Mitochondrial Membranes (drug effects, physiology)
  • Models, Molecular
  • Molecular Conformation

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