Abstract |
The objective of this study was to investigate the antiproliferative activity and apoptosis-inducing mechanism of Concanavalin A (ConA) on human melanoma A375 cells. We firstly simulated the three-dimensional structure of ConA. Subsequently, we found that ConA possessed remarkable antiproliferative effect on A375 cells. Further experimental data indicated that there was a link between its hemagglutinating activity, mannose-binding activity and antiproliferative activity. In addition, we showed that ConA induced A375 cell apoptosis in a caspase-dependent manner. Then, we demonstrated that the treatment of ConA caused mitochondrial transmembrane potential ( MMP) collapse, cytochrome c release and caspase activation. In conclusion, we report for the first time that there may be a close correlation between carbohydrate-binding activity of ConA and its antiproliferative activity. Also, we demonstrate firstly that ConA induces A375 cell death in a caspase-dependent manner as well as through a mitochondrial apoptotic pathway.
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Authors | Bo Liu, Chun-yang Li, He-jiao Bian, Ming-wei Min, Long-fei Chen, Jin-ku Bao |
Journal | Archives of biochemistry and biophysics
(Arch Biochem Biophys)
Vol. 482
Issue 1-2
Pg. 1-6
(Feb 2009)
ISSN: 1096-0384 [Electronic] United States |
PMID | 19111670
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Concanavalin A
- Caspase 3
- Caspase 8
- Caspase 9
- Mannose
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Topics |
- Apoptosis
(drug effects)
- Caspase 3
(metabolism)
- Caspase 8
(metabolism)
- Caspase 9
(metabolism)
- Cell Division
(drug effects)
- Cell Line, Tumor
- Computer Simulation
- Concanavalin A
(chemistry, pharmacology)
- Hemagglutination
(drug effects)
- Humans
- Mannose
(pharmacology)
- Melanoma
- Membrane Potentials
(drug effects)
- Mitochondrial Membranes
(drug effects, physiology)
- Models, Molecular
- Molecular Conformation
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