Abstract | OBJECTIVE: To investigate the effects of androstenedione on ovarian follicle development. DESIGN: Experimental study. SETTING: University research laboratory. ANIMAL(S): Female Wistar-Imamichi rats and BDF1 mice. INTERVENTION(S): MAIN OUTCOME MEASURE(S): RESULT(S): In androstenedione-treated rat ovaries, follicular cysts were formed, and apoptotic cells were found in the inner part of granulosa cell layers of antral follicles. Androstenedione administration down-regulated expression of P450arom but up-regulated expression of P450scc and p27(Kip1) in the granulosa cells of antral follicles. Serum T levels were significantly increased in androstenedione-treated rats. In mouse follicles exposed to androstenedione, the granulosa cells contained abundant lipid droplets and mitochondria with complex tubular cristae. CONCLUSION(S): Excess androgen enhances apoptosis in the inner part of granulosa cell layers of antral follicles, resulting in the formation of follicular cysts. It is also demonstrated that androgen stimulates premature luteinization of granulosa cells.
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Authors | Yuki Okutsu, Masanori T Itoh, Noriyuki Takahashi, Bunpei Ishizuka |
Journal | Fertility and sterility
(Fertil Steril)
Vol. 93
Issue 3
Pg. 927-35
(Feb 2010)
ISSN: 1556-5653 [Electronic] United States |
PMID | 19100535
(Publication Type: Journal Article)
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Copyright | Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Cdkn1b protein, mouse
- Cyclin-Dependent Kinase Inhibitor p27
- Androstenedione
- Cytochrome P-450 Enzyme System
- Aromatase
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Topics |
- Androstenedione
(pharmacology)
- Animals
- Apoptosis
(drug effects)
- Aromatase
(metabolism)
- Cell Communication
(drug effects)
- Cyclin-Dependent Kinase Inhibitor p27
(metabolism)
- Cytochrome P-450 Enzyme System
(metabolism)
- Female
- Follicular Cyst
(chemically induced, pathology)
- Granulosa Cells
(drug effects, ultrastructure)
- Luteinization
(drug effects)
- Mice
- Mice, Inbred Strains
- Microscopy, Electron, Transmission
- Oocytes
(drug effects, ultrastructure)
- Organ Culture Techniques
- Ovarian Follicle
(cytology, drug effects, growth & development)
- Polycystic Ovary Syndrome
(pathology)
- Rats
- Rats, Wistar
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