[Parathyroid hormone 1-34 induce cardiac myocytes hypertrophy via extracellular regulated protein kinase 1/2 pathway].

Abstract

OBJECTIVE:

To investigate the role of mitogen activated protein kinase kinase 1 (MAPKK, MEK1) and regulated kinase1/2 (ERK1/2) on cardiac hypertrophy induced by rat parathyroid hormone1-34 (rPTH1-34).

METHOD:

Neonatal rat cardiomyocytes was treated with or without 10(-7) mol/L rPTH1-34 in the absence or presence 2 x 10(-5) mol/L PD98059, a MEK1 inhibitor. Cellular diameter was measured by Motic Images Advanced 3.0 software and the synthetic rate of protein in cardiac myocytes was detected by 3H-leucine incorporation, mRNA expression of atrial natriuretic peptide (ANP) was measured by RT-PCR and protein expression of ERK1/2 and p-ERK1/2 was measured by Western blot.

RESULTS:

rPTH1-34 (10(-7) mol/L) significantly increase cellular diameter (+13.6 microm), 3H-leucine incorporation (+898 cpm/well), ANP mRNA expression (+73.9%), and p-ERK1/2 protein expression (+15%) compared to control cells (all P < 0.05) and these effects could be significantly attenuated by PD98059: cellular diameter (-7.1 microm), 3H-leucin e incorporation (-644 cpm/well), ANP mRNA expression (-52.2%), and protein expression of p-ERK1/2 (-18%) (all P < 0.05 vs. PTH group). PD98059 did not affect control cells without PTH treatment (all P > 0.05).

CONCLUSIONS:

PD98059 attenuates PTH induced cardiac hypertrophy in vitro via inhibiting the expression of ERK1/2 and p-ERK1/2.

• Authors: Xiao-gang Liu, Hui-xia An, Su-yan Liu, Ye-ping Ren, Wei-bing Zhang
• Journal: Zhonghua xin xue guan bing za zhi (Zhonghua Xin Xue Guan Bing Za Zhi) Vol. 36 Issue 5 Pg. 439-43 (May 2008) ISSN: 0253-3758 [Print] China
• PMID: 19100042 (Publication Type: Journal Article)

Chemical References

• Flavonoids
• Parathyroid Hormone
• Atrial Natriuretic Factor
• MAP Kinase Kinase 1
• MAP Kinase Kinase 2
• 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one

Topics

• Animals
• Atrial Natriuretic Factor (metabolism)
• Cardiomegaly (chemically induced, metabolism)
• Cells, Cultured
• Flavonoids (pharmacology)
• Gene Expression Regulation
• MAP Kinase Kinase 1 (metabolism)
• MAP Kinase Kinase 2 (metabolism)
• MAP Kinase Signaling System
• Myocytes, Cardiac (metabolism)
• Parathyroid Hormone (adverse effects)
• Rats
• Rats, Wistar