Effect of interleukin-10 overexpression on the properties of healing tendon in a murine patellar tendon model.

Abstract

PURPOSE:

Interleukin-10 (IL-10) is a potent anti-inflammatory cytokine shown to inhibit scar formation in fetal wound healing. The role of IL-10 in adult tendon healing and scar formation, however, remains unknown. The objective of this study is to investigate the effect of IL-10 overexpression on the properties of adult healing tendon using a well-established murine model of tendon injury and a lentiviral-mediated method of IL-10 overexpression.

METHODS:

A murine model of patellar tendon injury was used and animals divided into 3 groups. Mice received bilateral patellar tendon injections with a lentiviral vector containing an IL-10 transgene (n = 34) or no transgene (n = 34). Control mice (n = 34) received injections of sterile saline. All animals then were subjected to bilateral, central patellar tendon injuries 2 days after injection and were killed at 5, 10, 21, and 42 days after injury. IL-10 content was analyzed by immunohistochemistry (n = 4/group). Tendon healing was evaluated by histology (n = 4/group) and biomechanical analysis (n = 10/group).

RESULTS:

Overexpression of IL-10 in patellar tendon was confirmed after injection of the lentiviral vector. IL-10 immunostaining was increased at day 10 in the IL-10 group relative to that in controls. Histologically, there was no significant difference in angular deviation between groups at day 21, but a trend toward decreased angular deviation in controls relative to that in empty vector group mice was seen at day 42. Biomechanically, the IL-10 group showed significantly increased maximum stress at day 42 relative to that in controls. Percent relaxation showed a trend toward an increase at day 10 and a significant increase at day 42 in the IL-10 group relative to that in controls.

CONCLUSIONS:

This study demonstrates successful gene transfer of IL-10 into adult murine patellar tendon using a lentiviral vector. Although the effects of overexpression of IL-10 on adult tendon healing have not yet been fully elucidated, the current study may help to further clarify the mechanisms of tendon injury and repair.

• Authors: Eric T Ricchetti, Sudheer C Reddy, Heather L Ansorge, Miltiadis H Zgonis, Jonathan P Van Kleunen, Kenneth W Liechty, Louis J Soslowsky, Pedro K Beredjiklian
• Journal: The Journal of hand surgery (J Hand Surg Am) Vol. 33 Issue 10 Pg. 1843-52 (Dec 2008) ISSN: 1531-6564 [Electronic] United States
• PMID: 19084188 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-10

Topics

• Animals
• Disease Models, Animal
• Gene Transfer Techniques
• Genetic Therapy
• Genetic Vectors
• Interleukin-10 (physiology)
• Lentivirus
• Male
• Mice
• Patellar Ligament (injuries)
• Tendon Injuries (genetics, metabolism, therapy)
• Weight-Bearing
• Wound Healing (physiology)