[Long-term induced effects by only one treatment with ketoconazole on rat tumoral pituitary cells (GH3/B6 strains)].

Abstract

Ketoconazole, an imidazole is a powerful antimycotic that has recently been used in the treatment of endocrinological and lipid metabolism disorders as well as in anti-cancer chemotherapy. When rat mammosomatotropic cells are treated for 30 hrs., this drug produces different effects depending on whether cells are normal or tumoral (GH3/B6). A dose of 10 microM had no effect in normal cells, however, in tumoral cells it had dramatic effects: (i) 50% of the cells are killed and those which survive no longer proliferate and they secrete GH but not PRL; (ii) they respond to GHRH in a dose-dependent manner, while normal cells do not under similar culture conditions; (iii) the lipid composition of the membrane is modified as indicated by the increase in arachidonic acid turn-over and the dramatic change in the distribution of its metabolites. For the moment we cannot explain these data.

• Authors: R Drouhault, P Vacher, D Darret, J Larrue, A M Vacher, N Vilayleck
• Journal: Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie (C R Acad Sci III) Vol. 312 Issue 12 Pg. 615-22 ( 1991) ISSN: 0764-4469 [Print] France
• Vernacular Title: Effets induits à long terme par un seul traitement de kétoconazole sur des cellules tumorales hypophysaires de rat (lignée GH3/B6).
• PMID: 1907522 (Publication Type: English Abstract, Journal Article)

Chemical References

• Arachidonic Acids
• Thyrotropin-Releasing Hormone
• Growth Hormone-Releasing Hormone
• Ketoconazole

Topics

• Animals
• Arachidonic Acids (metabolism)
• Electrophysiology
• Growth Hormone-Releasing Hormone (pharmacology)
• Ketoconazole (pharmacology)
• Pituitary Gland (drug effects, metabolism, pathology, physiopathology)
• Pituitary Neoplasms (pathology, physiopathology)
• Rats
• Rats, Inbred Strains
• Thyrotropin-Releasing Hormone (pharmacology)