An improved method for the detection of differential survival between normal and xeroderma pigmentosum lymphoblastoid cell lines in culture with 4-nitroquinoline-1-oxide.

Abstract

The effects of the UV-mimetic chemical 4-nitroquinoline-1-oxide (4-NQO) upon cell lines heterozygous or homozygous for the recessive mutant xeroderma pigmentosum (XP) were investigated. Human lymphoblastoid cell lines, which were established from 4 XP homozygote patients (XPL15, XPL17, XPL19 and XPL20). 2 XP heterozygote individuals (XPPL17 and XPML17) and 58 normal individuals, were cultured in the presence of 4-NQO at doses of 0, 2, 4 and 8 x 10(-6) M. Then the total cell number was counted and the viability of the cells was measured by the dye exclusion method using trypan blue and a newly devised fluorometric method with fluorescein diacetate. Results showed that 4-NQO affected, in increasing order of impairment, the cell lines: normal less than XP heterozygote less than XP homozygote.

• Authors: C Kiyohara, T Hirohata, M Kuratsune, J Nagayama
• Journal: Mutation research (Mutat Res) Vol. 249 Issue 1 Pg. 111-7 (Jul 1991) ISSN: 0027-5107 [Print] Netherlands
• PMID: 1906129 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 4-Nitroquinoline-1-oxide

Topics

• 4-Nitroquinoline-1-oxide (toxicity)
• Cell Division (drug effects)
• Cell Line
• Cell Survival (drug effects)
• Heterozygote
• Homozygote
• Humans
• Leukemia, Lymphoid (pathology)
• Mutation
• Xeroderma Pigmentosum (genetics, pathology)