Abstract |
We examined the in vitro effects of imatinib (Novartis Pharma AG, Basel, Switzerland) as a possible inhibitor of PDGFR pathway on cells derived from a recurrence of a pleural malignant solitary fibrous tumor (SFT). Primary cell culture was characterised by immunofluorescence. SFT-derived cells were treated with imatinib at different time points. Western blotting for PDGFR-beta, phospho- PDGFR-beta or smooth muscle actin (SMA) was performed before and after 96 h of treatment with imatinib. SFT-derived cells treated with imatinib for 96 h showed a dose dependent decrease of Ki67 expression. Results were confirmed by growth curve. Western blotting showed that PDGFR-beta was highly expressed and phosphorylated in SFT-derived cells and imatinib treatment reduced PDGFR-beta phosphorylation and SMA expression. With the limit of experimental findings, our results support a possible future application of imatinib as a candidate molecule in the target therapy of malignant SFTs over-expressing wild-type PDGFR.
|
Authors | Marco Prunotto, Martino Bosco, Lorenzo Daniele, Luigia Macri', Lisa Bonello, Laura Schirosi, Giulio Rossi, Pierluigi Filosso, Baudolino Mussa, Anna Sapino |
Journal | Lung cancer (Amsterdam, Netherlands)
(Lung Cancer)
Vol. 64
Issue 2
Pg. 244-6
(May 2009)
ISSN: 1872-8332 [Electronic] Ireland |
PMID | 19041155
(Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antineoplastic Agents
- Benzamides
- Piperazines
- Pyrimidines
- Imatinib Mesylate
- Receptor, Platelet-Derived Growth Factor alpha
- Receptor, Platelet-Derived Growth Factor beta
- Cisplatin
- Fluorouracil
|
Topics |
- Aged
- Antineoplastic Agents
(pharmacology)
- Antineoplastic Combined Chemotherapy Protocols
(therapeutic use)
- Benzamides
- Blotting, Western
- Cell Proliferation
(drug effects)
- Cells, Cultured
- Cisplatin
(administration & dosage)
- Female
- Fluorescent Antibody Technique
- Fluorouracil
(administration & dosage)
- Humans
- Imatinib Mesylate
- In Vitro Techniques
- Neoplasm Recurrence, Local
(metabolism, pathology)
- Piperazines
(pharmacology)
- Pneumonectomy
- Pyrimidines
(pharmacology)
- Radiotherapy
- Receptor, Platelet-Derived Growth Factor alpha
(biosynthesis)
- Receptor, Platelet-Derived Growth Factor beta
(biosynthesis, drug effects)
- Solitary Fibrous Tumor, Pleural
(metabolism, pathology, therapy)
|