Characterization of a novel type of chain-terminator Gal beta 1-6Gal beta 1-4)GlcNAc in an oligosaccharide related to N-glycosylated protein glycans isolated from GM1 the urine of patients with gangliosidosis.

Abstract

Two new oligosaccharides were isolated from the urine of a patient with GM1 gangliosidosis. Final purification of the oligosaccharides was accomplished by capillary supercritical fluid chromatography. Structural analysis was by chemical analysis, chemical-ionization mass spectrometry and 400-MHz 1H-NMR spectroscopy, leading to two primary structures. The first is derived from a classical triantennary N-acetyllactosamine-type glycan: Gal beta 1-4GlcNAc beta 1-4(Gal beta 1-4GlcNAc beta 1-2)Man alpha 1-3Man beta 1-4GlcNAc. The second is unusual with a terminal disaccharide Gal beta 1-6Gal, which had not yet been described for glycans of the N-acetyllactosamine type: Gal beta 1-6Gal beta 1-4GlcNAc beta 1-2Man alpha 1-6Man beta 1-4GlcNAc.

• Authors: J C Michalski, J Lemoine, J M Wieruszeski, B Fournet, J Montreuil, G Strecker
• Journal: European journal of biochemistry (Eur J Biochem) Vol. 198 Issue 2 Pg. 521-6 (Jun 01 1991) ISSN: 0014-2956 [Print] England
• PMID: 1904026 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Oligosaccharides
• Proteoglycans
• G(M1) Ganglioside

Topics

• Carbohydrate Conformation
• Carbohydrate Sequence
• Chromatography, Paper
• G(M1) Ganglioside (isolation & purification, urine)
• Gangliosidoses (urine)
• Glycosylation
• Humans
• Magnetic Resonance Spectroscopy
• Mass Spectrometry
• Molecular Sequence Data
• Oligosaccharides (isolation & purification, urine)
• Proteoglycans (isolation & purification, urine)