Helicobacter pylori infection and expression of DNA mismatch repair proteins.

Abstract

AIM:

To determine the expression of DNA (MMR) proteins, including hMLH1 and hMSH2, in gastric epithelial cells in the patients with or without Helicobacter pylori (H pylori)-infected gastritis.

METHODS:

Fifty H pylori-positive patients and 50 H pylori-negative patients were enrolled in the study. During endoscopy of patients with non-ulcer dyspepsia, two antral and two corpus biopsies were taken for histological examination (Giemsa stain) and for immunohistochemical staining of hMLH1 and hMSH2.

RESULTS:

The percentage of epithelial cell nuclei that demonstrated positivity for hMLH1 staining was 84.14 +/- 7.32% in H pylori-negative patients, while it was 73.34 +/- 10.10% in H pylori-positive patients (P < 0.0001). No significant difference was seen between the two groups regarding the percentage of epithelial cell nuclei that demonstrated positivity for hMSH2 staining (81.16 +/- 8.32% in H pylori-negative versus 78.24 +/- 8.71% in H pylori-positive patients; P = 0.09).

CONCLUSION:

This study indicates that H pylori might promote development of gastric carcinoma at least in part through its ability to affect the DNA MMR system.

• Authors: Vahid Mirzaee, Mahsa Molaei, Hamid-Mohaghegh Shalmani, Mohammad-Reza Zali
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 14 Issue 43 Pg. 6717-21 (Nov 21 2008) ISSN: 1007-9327 [Print] United States
• PMID: 19034977 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Adaptor Proteins, Signal Transducing
• MLH1 protein, human
• Neoplasm Proteins
• Nuclear Proteins
• PMS1 protein, human
• MutL Protein Homolog 1
• MutL Proteins

Topics

• Adaptor Proteins, Signal Transducing (metabolism)
• Adult
• Biopsy
• DNA Mismatch Repair (physiology)
• Epithelium (metabolism, pathology)
• Female
• Gastric Mucosa (metabolism)
• Gastritis (metabolism, pathology)
• Helicobacter Infections (metabolism, pathology)
• Helicobacter pylori
• Humans
• Male
• Microsatellite Instability
• Middle Aged
• MutL Protein Homolog 1
• MutL Proteins
• Neoplasm Proteins (metabolism)
• Nuclear Proteins (metabolism)
• Stomach (pathology)