Abstract | BACKGROUND: omega-5 gliadin is a major allergen in exercise-induced wheat allergy (EIWA), but it is also implicated in immediate-type reactions to wheat. An ImmunoCAP assay to measure omega-5 gliadin-specific IgE has become available. This study aimed to evaluate this new biological test in wheat allergy diagnosis and to also determine if it was able to discriminate EIWA from other types of wheat allergy. METHODS: Sixty-one patients with wheat allergy were divided into 3 groups as a function of their symptoms (EIWA, immediate-type reactions and atopic dermatitis). These patients underwent skin prick tests with purified omega gliadins and ImmunoCAP to wheat flour, gluten and recombinant omega-5 gliadin. RESULTS: The experimental data showed that 78% of EIWA patients had a positive skin prick test to natural omega-5 gliadin and the same proportion had detectable specific IgE to recombinant omega-5 gliadin, indicating that omega-5 gliadin is the main allergen, but not the only one, in our population. Additionally, we showed that this detection was not EIWA specific since omega-5 gliadin-specific IgE was detected in 30% of other patients who had a wheat allergy. These results lead to a positive predictive value of 37.5% and to a negative predictive value of 91%. CONCLUSIONS:
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Authors | S Jacquenet, M Morisset, F Battais, S Denery-Papini, A Croizier, E Baudouin, B Bihain, D A Moneret-Vautrin |
Journal | International archives of allergy and immunology
(Int Arch Allergy Immunol)
Vol. 149
Issue 1
Pg. 74-80
( 2009)
ISSN: 1423-0097 [Electronic] Switzerland |
PMID | 19033710
(Publication Type: Journal Article)
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Copyright | 2008 S. Karger AG, Basel. |
Chemical References |
- Allergens
- Antigens, Plant
- Recombinant Proteins
- omega-5 gliadin, wheat
- Immunoglobulin E
- Gliadin
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Topics |
- Adolescent
- Adult
- Aged
- Allergens
(immunology)
- Antigens, Plant
- Child
- Child, Preschool
- Exercise
- Female
- Gliadin
(immunology)
- Humans
- Immunoassay
- Immunoglobulin E
(blood)
- Infant
- Male
- Middle Aged
- Recombinant Proteins
(immunology)
- Skin Tests
- Wheat Hypersensitivity
(diagnosis, immunology)
- Young Adult
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