Abstract | AIM: METHODS: A film dispersion method was used to prepare the PE-sub liposome, an orthogonal analysis was conducted to optimize the PE-sub liposome preparation condition and determine the effects of liposomes as carriers on cell uptake in vitro. Under a fluorescence microscope, the cell survival rate of normal liver cell line HL7702 and liver cancer cell line HepG2 was assessed by 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide assay. Cell apoptosis was determined with flow cytometry and acridine orange staining after PDT treatment. RESULTS: The optimum preparation conditions of the PE-sub liposome were found: a phosphatidylcholine-to-cholesterin ratio of 1:2, a PE-sub-to- lipid ratio of 1:30, 20 mL buffer volume, 10 min sonication time, and an average encapsulation rate of up to 47.2%. The particle size ranged from 80 to 200 nm, and the average particle diameter was 136 nm. At a concentration of 100 microg/mL, the transfection rate of the PE-sub liposome reached 18% at 2 h and 24% at 4 h, and remained steady at 5-6 h. The half lethal dose of PDT on HepG2 was 75 microg/mL, whereas the cell survival rate of HL7702 reached 80% at the same dosage. The PDT-treated cells showed characteristics of apoptosis. CONCLUSION: The film dispersion method was found to maintain the biological characteristics of the PE-sub. The use of the liposome carrier increased the PE-sub accumulation in the cells and enhanced its PDT effect on HepG2 compared to the PE-sub. HL7702 cell toxicity on had less apparent change after PDT treatment. The PE-sub liposome demonstrated good tumor-targeting characteristics in the in vitro experiment.
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Authors | Ling Hu, Bei Huang, Man-man Zuo, Rui-yong Guo, Hao Wei |
Journal | Acta pharmacologica Sinica
(Acta Pharmacol Sin)
Vol. 29
Issue 12
Pg. 1539-46
(Dec 2008)
ISSN: 1745-7254 [Electronic] United States |
PMID | 19026175
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Drug Carriers
- Liposomes
- Protein Subunits
- Phycoerythrin
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Topics |
- Cell Line, Tumor
- Drug Carriers
(chemistry)
- Humans
- Liposomes
(chemistry)
- Liver Neoplasms
(therapy)
- Particle Size
- Photochemotherapy
(methods)
- Phycoerythrin
(chemistry, metabolism, therapeutic use)
- Protein Subunits
(chemistry, metabolism, therapeutic use)
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