[Study on the susceptibility of the cervical carcinoma cells to CTL lysis under the stimulation of rSIFN-co and its mechanism].

Abstract

OBJECTIVE:

To investigate the susceptibility of cervical carcinoma cells (HPV16+) to CTL lysis affected by rSIFN-co, consensus Interferon (Infergen), IFNalpha-2b and DDP.

METHODS:

After CaSki cervical cancer cells were induced by rSIFN-co, Infergen, IFNalpha-2b and DDP at the concentration of 0.156 microg/mL, 0.625 microg/mL, 2.500 microg/mL for 72 hours, CaSki cells which had been induced were effected by CTL, the cytotoxicity was determined and calculated by MTT assay. The expression intensity of adhesion molecules on Caski cell such as CD54 and CD40 was also determined by flow cytometry.

RESULTS:

The susceptibility of CaSki cell to CTL lysis under the stimulation of rSIFN-co was better than Infergen, IFNalpha-2b and DDP induced. The expression of CD54 and CD40 on cervical cancer cell was also increased. And this effect had positive correlation to the drug concentration.

CONCLUSION:

rSIFN-co can increase the expression of CD54 and CD40 on the cervical cancer cell surface, and increase the susceptibility of CaSki cell to specific effective cell lysis in a dose-dependent manner. The effect of rSIFN-co is better than same type interferon, general I type interferon and chemotherapeutic drug induced.

• Authors: Yan Chen, Yue-dong He, Xiao-ling Pan, Xia Liu, Zeng-zhen Lai
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 39 Issue 5 Pg. 715-8 (Sep 2008) ISSN: 1672-173X [Print] China
• PMID: 19024297 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CD40 Antigens
• Recombinant Proteins
• Intercellular Adhesion Molecule-1
• Interferon-gamma

Topics

• CD40 Antigens (metabolism)
• Cytotoxicity, Immunologic (immunology)
• Female
• Humans
• Intercellular Adhesion Molecule-1 (metabolism)
• Interferon-gamma (pharmacology)
• Recombinant Proteins
• T-Lymphocytes, Cytotoxic (immunology)
• Tumor Cells, Cultured
• Uterine Cervical Neoplasms (immunology, pathology)