Abstract | OBJECTIVES: The aim of this study was to evaluate the effect of myoblast transplantation on left ventricular function, perfusion, and scar formation after compromised coronary flow. DESIGN: A coronary vessel with Ameroid-induced stenosis was ligated and skeletal muscle was biopsied for isolation and cultivation of myoblasts. Two weeks after ligation, animals were randomly selected to receive intramyocardial injections of 2 x 10(6) myoblasts or vehicle. Fifteen animals survived the whole study period (n=9 and n=6, respectively). All animals underwent cardiac magnetic resonance imaging (MRI) and angiography pretreatment and four weeks posttreatment. RESULTS: Peak filling rate of the left ventricle improved in the myoblast group (p=0.0048), but not in the control group. Peak ejection rate and duration of diastole improved only in the myoblast group (p=0.049 and p=0.0039, respectively). Ejection fraction or local thickening did not change. Fibrosis and perfusion were similar in both groups, but more microvessels were present histologically in the myoblast group. CONCLUSIONS: In this preclinical study, autologous myoblast transplantation improved ischemic heart function via enhanced diastolic filling of the left ventricle.
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Authors | Tommi Pätilä, Tuija Ikonen, Esko Kankuri, Aki Uutela, Jyri Lommi, Leena Krogerus, Pertteli Salmenperä, Josef Bizik, Kirsi Lauerma, Ari Harjula |
Journal | Scandinavian cardiovascular journal : SCJ
(Scand Cardiovasc J)
Vol. 43
Issue 2
Pg. 100-9
(Apr 2009)
ISSN: 1651-2006 [Electronic] England |
PMID | 19016109
(Publication Type: Journal Article)
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Topics |
- Animals
- Cells, Cultured
- Coronary Angiography
- Coronary Circulation
- Disease Models, Animal
- Fibrosis
- Magnetic Resonance Imaging
- Microcirculation
- Myoblasts, Skeletal
(transplantation)
- Myocardial Contraction
- Myocardial Infarction
(etiology, pathology, physiopathology, surgery)
- Myocardial Ischemia
(complications, pathology, physiopathology, surgery)
- Myocardium
(pathology)
- Stroke Volume
- Swine
- Time Factors
- Transplantation, Autologous
- Ventricular Function, Left
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