Betaig-h3 interacts with alpha3beta1 integrin to promote adhesion and migration of human hepatoma cells.

Abstract

betaig-h3 is a TGF-induced extracellular matrix (ECM) protein. Our previous evidence suggests that beta ig-h3 may promote adhesion and invasion potential of human hepatoma cells, but the mechanism underlying this process is still unknown. The present study identifies a pivotal role for molecules of the beta ig-h3 signal transduction pathway. We demonstrated that beta ig-h3 co-immunoprecipitated with alpha 3beta 1 integrin in human 7721 hepatoma cells. The addition of alpha 3beta 1 integrin antibodies inhibited the elevated adhesion and migration in beta ig-h3-over-expressing 7721 cells, but not in beta ig-h3 siRNA transfected 7721 cells. The expression and activity of integrin downstream molecules FAK and paxillin show a positive correlation with beta ig-h3 expression in different human hepatoma cells. Levels of focal adhesions and stress fibers were decreased in beta ig-h3 siRNA transfected 7721 cells. We suggest that by interaction with alpha 3beta 1 integrin, beta ig-h3 activates FAK-paxillin signaling linkage, leads to cytoskeleton reorganization, and thus enhances the metastatic potentials of human hepatoma cells.

• Authors: Juan Tang, Ya-Mei Wu, Pu Zhao, Jian-Li Jiang, Zhi-Nan Chen
• Journal: Experimental biology and medicine (Maywood, N.J.) (Exp Biol Med (Maywood)) Vol. 234 Issue 1 Pg. 35-9 (Jan 2009) ISSN: 1535-3702 [Print] England
• PMID: 18997105 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Extracellular Matrix Proteins
• Integrin alpha3beta1
• Paxillin
• Transforming Growth Factor beta
• betaIG-H3 protein
• Focal Adhesion Kinase 1
• PTK2 protein, human

Topics

• Carcinoma, Hepatocellular (pathology, physiopathology)
• Cell Adhesion
• Cell Line, Tumor
• Cell Movement
• Cytoskeleton (pathology, ultrastructure)
• Extracellular Matrix Proteins (genetics, metabolism)
• Focal Adhesion Kinase 1 (genetics)
• Gene Expression Regulation, Neoplastic
• Gene Silencing
• Humans
• Integrin alpha3beta1 (genetics, metabolism)
• Liver Neoplasms (pathology, physiopathology)
• Neoplasm Invasiveness
• Paxillin (genetics)
• Transfection
• Transforming Growth Factor beta (genetics, metabolism)