Abstract | ETHNOPHARMACOLOGICAL RELEVANCE: Tripterygium wilfordii Hook. f. (Celastraceae) has been traditionally used as folk medicine for centuries in China for the treatment of immune-inflammatory diseases. AIM OF THE STUDY: This study aimed to assess the antiangiogenic activities which support the therapeutic use of Tripterygium wilfordii and its terpenoids for angiogenesis disease such as cancer. MATERIALS AND METHODS: The ethanol extract of Tripterygium wilfordii and subsequent fractions were evaluated on an in vivo antiangiogenic zebrafish embryo model. RESULTS: Three antiangiogenic terpenoids were isolated by bioassay-guided purification, namely, celastrol (4), cangoronine (5) and triptolide (7). Among them, triptolide manifested the most potent antiangiogenic activity against vessel formation by nearly 50% at 1.2 microM. Semi-quantitative RT-PCR analysis revealed that triptolide dose- and time-dependently reduced the mRNA expression of angiopoietin (angpt)2 and tie2 in zebrafish, indicating the involvement of angpt2/tie2 signaling pathway in the antiangiogenic action of triptolide. CONCLUSIONS: The discovery of an alternative pathway further confirms the value of ethnopharmacological investigations into traditional botanicals for leads for potential drug development.
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Authors | Ming-Fang He, Lin Liu, Wei Ge, Pang-Chui Shaw, Renwang Jiang, Li-Wha Wu, Paul Pui-Hay But |
Journal | Journal of ethnopharmacology
(J Ethnopharmacol)
Vol. 121
Issue 1
Pg. 61-8
(Jan 12 2009)
ISSN: 0378-8741 [Print] Ireland |
PMID | 18996177
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Angiogenesis Inhibitors
- Diterpenes
- Drugs, Chinese Herbal
- Epoxy Compounds
- Pentacyclic Triterpenes
- Phenanthrenes
- Terpenes
- Triterpenes
- cangoronine
- triptolide
- celastrol
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Topics |
- Angiogenesis Inhibitors
(chemistry, pharmacology)
- Animals
- Disease Models, Animal
- Diterpenes
(chemistry, pharmacology)
- Drugs, Chinese Herbal
(pharmacology)
- Embryo, Nonmammalian
(blood supply)
- Epoxy Compounds
(chemistry, pharmacology)
- Neovascularization, Physiologic
(drug effects)
- Pentacyclic Triterpenes
- Phenanthrenes
(chemistry, pharmacology)
- Plant Roots
(chemistry)
- Reverse Transcriptase Polymerase Chain Reaction
- Terpenes
(chemistry, pharmacology)
- Tripterygium
(chemistry)
- Triterpenes
(chemistry, pharmacology)
- Zebrafish
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