Abstract |
The aim of this study was to compare fibulin-5 expression in women with and without anterior vaginal wall prolapse. Vaginal tissues were sampled in a standardized fashion from women with (n = 12) or without (n = 10) anterior vaginal wall prolapse. Quantitative real-time polymerase chain reaction was performed to measure mRNA levels of fibulin-5 (FIB-5). FIB-5 protein expression was assessed by immunohistochemistry. There were no significant differences in demographic data between the two groups. FIB-5 mRNA expression was significantly decreased in women with anterior vaginal wall prolapse compared to women without prolapse [(FIB-5 mean +/- SD mRNA expression in relative units) 0.01 +/- 0.01 vs. 0.09 +/- 0.14, P = 0.04]. Fibulin-5 staining intensity was diminished in women with prolapse compared to women without prolapse [intensity score, median (range), 1 (1-2) vs. 3 (2-3), P = 0.04]. Fibulin-5 expression is decreased in vaginal biopsies from women with prolapse. Changes in fibulin expression may play a role in the development of pelvic organ prolapse.
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Authors | Peter Takacs, Mehdi Nassiri, Anita Viciana, Keith Candiotti, Alessia Fornoni, Carlos A Medina |
Journal | International urogynecology journal and pelvic floor dysfunction
(Int Urogynecol J Pelvic Floor Dysfunct)
Vol. 20
Issue 2
Pg. 207-11
(Feb 2009)
England |
PMID | 18989607
(Publication Type: Journal Article)
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Chemical References |
- Extracellular Matrix Proteins
- FBLN5 protein, human
- RNA, Messenger
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Topics |
- Biopsy
- Case-Control Studies
- Extracellular Matrix Proteins
(metabolism)
- Female
- Humans
- Hysterectomy
- Middle Aged
- RNA, Messenger
(metabolism)
- Uterine Prolapse
(etiology, metabolism, physiopathology)
- Vagina
(metabolism, pathology)
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