Control of chronic otitis media and sensorineural hearing loss in C3H/HeJ mice: glucocorticoids vs mineralocorticoids.

Abstract

OBJECTIVE:

The impact of glucocorticoids and mineralocorticoids on chronic otitis media (COM) in toll-like receptor 4-deficient C3H/HeJ mice was investigated.

STUDY DESIGN:

To evaluate control of COM by steroids with differences in their anti-inflammatory (prednisolone, dexamethasone), and fluid absorption functions (fludrocortisone, aldosterone). A minimum sample size of five animals for each group was required based on power analysis calculations. Sample sizes ranged from 7 to 17 mice per treatment group.

SUBJECTS AND METHODS:

Auditory brain stem response (ABR) thresholds were performed at baseline, 2 weeks and 4 weeks. Histopathologic test results were evaluated on all mice ears at the end of the study.

RESULTS:

Analysis of variance (ANOVA) of ABR threshold change showed significant treatment effects (P < 0.05) by both steroid types at all time intervals and ABR frequencies except 4 weeks/8 kHz. Histologic assessment showed prednisolone-treated mice (62%) had a higher rate of clearance of middle and inner ear inflammation than control mice (4%).

CONCLUSION:

It was concluded that steroid treatments can improve the physiology of chronic middle and inner ear disease seen with COM.

• Authors: Carol J MacArthur, J Beth Kempton, Jacqueline DeGagne, Dennis R Trune
• Journal: Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery (Otolaryngol Head Neck Surg) Vol. 139 Issue 5 Pg. 646-53 (Nov 2008) ISSN: 0194-5998 [Print] England
• PMID: 18984258 (Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Anti-Inflammatory Agents
• Glucocorticoids
• Mineralocorticoids
• Aldosterone
• Dexamethasone
• Prednisolone
• Fludrocortisone

Topics

• Aldosterone (therapeutic use)
• Animals
• Anti-Inflammatory Agents (therapeutic use)
• Chronic Disease
• Dexamethasone (therapeutic use)
• Evoked Potentials, Auditory, Brain Stem
• Fludrocortisone (therapeutic use)
• Glucocorticoids (therapeutic use)
• Hearing Loss, Sensorineural (etiology, physiopathology, prevention & control)
• Mice
• Mice, Inbred C3H
• Mineralocorticoids (therapeutic use)
• Otitis Media (complications, drug therapy, pathology)
• Prednisolone (therapeutic use)