Abstract |
The purpose of this study was to evaluate the pattern of the expression of orotate phosphoribosyltransferase (OPRT) in several components of colorectal carcinoma (CRC). Fifty-six surgically-resected samples of CRC were subjected to immunohistochemistry with a polyclonal anti-OPRT antibody. Grading was performed independently for several components of CRC, including mucosal carcinoma lesions (n=56), infiltrative lesions (n=53), lymphovascularly invasive lesions (n=34) and metastatic lymph nodes (n=17). The expression of OPRT in mucosal carcinoma and infiltrative lesions correlated significantly only with the presence of lymphovascular invasion (p=0.0007 and <0.0001, respectively). The frequency of OPRT expression in mucosal carcinoma, infiltrative and lymphovascularly invasive lesions as well as metastatic lymph nodes was 32.1, 69.8, 88.2 and 88.8%, respectively. In addition, nuclear staining of OPRT was observed in metastatic lymph nodes and lymphovascularly invasive lesions. Our results suggest that OPRT is involved in the invasion and metastasis of CRC.
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Authors | Yuichi Sanada, Kazuhiro Yoshida, Jun Hihara, Morihito Okada |
Journal | Oncology reports
(Oncol Rep)
Vol. 20
Issue 5
Pg. 1005-11
(Nov 2008)
ISSN: 1021-335X [Print] Greece |
PMID | 18949394
(Publication Type: Journal Article)
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Chemical References |
- CLDN1 protein, human
- Claudin-1
- Membrane Proteins
- Orotate Phosphoribosyltransferase
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Topics |
- Adenocarcinoma
(enzymology, pathology)
- Adult
- Aged
- Aged, 80 and over
- Claudin-1
- Colorectal Neoplasms
(enzymology, pathology)
- Female
- Humans
- Immunohistochemistry
- Lymphatic Metastasis
(pathology)
- Male
- Membrane Proteins
(biosynthesis)
- Middle Aged
- Orotate Phosphoribosyltransferase
(biosynthesis)
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