Effects of melatonin on spermatogenesis and testicular ischemia-reperfusion injury after unilateral testicular torsion-detorsion.

Abstract

PURPOSE:

This study aimed to determine the effect of melatonin in preventing ischemia-reperfusion (I-R) injury-induced tissue damage and on spermatogenesis after experimental testicular torsion (TT).

METHODS:

Forty peripubertal rats were divided into 4 groups each containing 10 rats: control (C), sham (S), torsion plus detorsion (TD), and torsion plus melatonin (M). The left testes were rotated 720 degrees for 6 hours and detorsed for 6 hours thereafter. Serum inhibin B (IB) levels were measured in blood samples taken from all groups. Left orchiectomies were performed to determine the tissue levels of Johnsen's scores (JS) and malondialdehyde (MDA).

RESULTS:

Serum IB levels in the S and TD groups were significantly lower compared with that in the C group, whereas they were higher in the M group compared with the TD group. The MDA levels were significantly lower in the C, S, and, M groups compared with the TD group. Johnsen's scores were significantly higher in the C, S, and M groups compared with the TD group.

CONCLUSIONS:

Our results suggested that melatonin is a potent antioxidant agent in preventing testicular I-R injury, as shown by increased IB levels and JS.

• Authors: Müslim Yurtçu, Adnan Abasiyanik, Mustafa Cihat Avunduk, Sabahattin Muhtaroğlu
• Journal: Journal of pediatric surgery (J Pediatr Surg) Vol. 43 Issue 10 Pg. 1873-8 (Oct 2008) ISSN: 1531-5037 [Electronic] United States
• PMID: 18926224 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antioxidants
• Biomarkers
• Free Radical Scavengers
• inhibin B
• Malondialdehyde
• Inhibins
• Glutathione Peroxidase
• Melatonin

Topics

• Animals
• Antioxidants (therapeutic use)
• Biomarkers
• Drug Evaluation, Preclinical
• Free Radical Scavengers (therapeutic use)
• Glutathione Peroxidase (metabolism)
• Inhibins (blood)
• Male
• Malondialdehyde (analysis)
• Melatonin (therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (blood, etiology, pathology, prevention & control)
• Rotation
• Severity of Illness Index
• Sperm Count
• Spermatic Cord Torsion (pathology, surgery)
• Spermatogenesis (drug effects)
• Testis (blood supply, chemistry, pathology)