Abstract |
Epidermodysplasia verruciformis (EV) is a rare disease, characterized by cutaneous warts and associated with a strong predisposition to beta-genus human papillomavirus (HPV). Earlier studies reported high copy numbers of HPV- DNA in nearly all skin tumors from EV patients, but neither HPV replication status in non-lesional skin nor anti-HPV seroreactivity in these patients have been reported yet. We therefore performed a comprehensive viral load analysis for the more common beta-HPV types on skin samples and plucked eyebrow hairs from four EV patients treated at our dermatology department. The results clearly demonstrate that they carry a multiplicity (up to eighteen types) of beta-HPV genotypes in both skin sites. Worthy of note, a high intrapatient concordance for specific types between hair bulbs and skin biopsies was observed and the same beta-PV profile was maintained over time. Viral load analysis revealed a load range between less than one HPV- DNA copy per 100 cells to more than 400 HPV- DNA copies per cell in both eyebrow hairs and skin proliferative lesions. Evaluation of seroreactivity to beta-HPV types in the four EV patients revealed that antibodies against the 16 beta-HPV were significantly more prevalent and showed higher titers than in the controls.
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Authors | Valentina Dell'Oste, Barbara Azzimonti, Marco De Andrea, Michele Mondini, Elisa Zavattaro, Giorgio Leigheb, Sönke J Weissenborn, Herbert Pfister, Kristina M Michael, Tim Waterboer, Michael Pawlita, Ada Amantea, Santo Landolfo, Marisa Gariglio |
Journal | The Journal of investigative dermatology
(J Invest Dermatol)
Vol. 129
Issue 4
Pg. 1026-34
(Apr 2009)
ISSN: 1523-1747 [Electronic] United States |
PMID | 18923444
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Viral
- DNA, Viral
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Topics |
- Adult
- Antibodies, Viral
(blood)
- Betapapillomavirus
(classification, immunology, isolation & purification)
- DNA, Viral
(analysis)
- Epidermodysplasia Verruciformis
(immunology, virology)
- Humans
- In Situ Hybridization
- Male
- Middle Aged
- Viral Load
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