Abstract | AIM: METHODS: Male SD rats were randomly divided into model group, protective groups A and B, and normal control group. Rats in the model group received only intra-colonic TNB. Rats in the protective groups A and B received intra-peritoneal ilomastat of 10 mg/kg and 20 mg/kg, respectively, beside TNB. Rats in the normal control group received only intra-colonic normal saline. After 3 wk, segments of colon were obtained. RT-PCR and immunohistochemistry were used to examine the expression of MMP-1 and TIMP-1. Hematoxylin- eosin (HE) staining was used for pathological study. RESULTS: The model of UC was successfully induced in rats. Inflammation of colonic mucosa greatly improved in protective groups A and B. Expression of MMP-1 and TIMP-1 in the model group, protective groups A and B was significantly higher than that in the normal control group (P < 0.0001) with MMP-1 expression increased more significantly than TIMP-1 expression. Expression of MMP-1 in protective groups A and B was significantly lower than that in the model group (P < 0.0001) . Expression of MMP-1 in protective group B was significantly lower than that in protective group A (P < 0.0001). CONCLUSION:
Ilomastat improves TNB-induced UC in rats by inhibiting the MMP-1 activity.
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Authors | Ying-De Wang, Wei Wang |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 14
Issue 37
Pg. 5683-8
(Oct 07 2008)
ISSN: 1007-9327 [Print] United States |
PMID | 18837084
(Publication Type: Journal Article)
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Chemical References |
- Anti-Inflammatory Agents
- Gastrointestinal Agents
- Hydroxamic Acids
- Indoles
- Matrix Metalloproteinase Inhibitors
- Protease Inhibitors
- RNA, Messenger
- Tissue Inhibitor of Metalloproteinase-1
- Trinitrobenzenesulfonic Acid
- Matrix Metalloproteinase 1
- ilomastat
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Topics |
- Animals
- Anti-Inflammatory Agents
(pharmacology)
- Colitis, Ulcerative
(chemically induced, enzymology, prevention & control)
- Colon
(drug effects, enzymology, pathology)
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Gastrointestinal Agents
(pharmacology)
- Hydroxamic Acids
- Immunohistochemistry
- Indoles
(pharmacology)
- Male
- Matrix Metalloproteinase 1
(genetics, metabolism)
- Matrix Metalloproteinase Inhibitors
- Protease Inhibitors
(pharmacology)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Reverse Transcriptase Polymerase Chain Reaction
- Tissue Inhibitor of Metalloproteinase-1
(metabolism)
- Trinitrobenzenesulfonic Acid
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