Metabolic urinary profiling of alcohol hepatotoxicity and intervention effects of Yin Chen Hao Tang in rats using ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry.

This paper was designed to study metabonomic characters of the hepatotoxicity induced by alcohol and the intervention effects of Yin Chen Hao Tang (YCHT), a classic traditional Chinese medicine formula for treatment of jaundice and liver disorders in China. Urinary samples from control, alcohol- and YCHT-treated rats were analyzed by ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry (UPLC/ESI-QTOF-MS) in positive ionization mode. The total ion chromatograms obtained from the control, alcohol- and YCHT-treated rats were easily distinguishable using a multivariate statistical analysis method such as the principal components analysis (PCA). The greatest difference in metabolic profiling was observed from alcohol-treated rats compared with the control and YCHT-treated rats. The positive ions m/z 664.3126 (9.00 min) was elevated in urine of alcohol-treated rats, whereas, ions m/z 155.3547 (10.96 min) and 708.2932 (9.01 min) were at a lower concentration compared with that in urine of control rats, however, these ions did not indicate a statistical difference between control rats and YCHT-treated rats. The ion m/z 664.3126 was found to correspond to ceramide (d18:1/25:0), providing further support for an involvement of the sphingomyelin signaling pathway in alcohol hepatotoxicity and the intervention effects of YCHT.
AuthorsXijun Wang, Haitao Lv, Hui Sun, Lian Liu, Bo Yang, Wenjun Sun, Ping Wang, Dixin Zhou, Li Zhao, Shengshan Dou, Guangmei Zhang, Hongxin Cao
JournalJournal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal) Vol. 48 Issue 4 Pg. 1161-8 (Dec 1 2008) ISSN: 0731-7085 [Print] England
PMID18835123 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Drugs, Chinese Herbal
  • Ethanol
  • Animals
  • Chromatography, High Pressure Liquid (methods)
  • Drug-Related Side Effects and Adverse Reactions
  • Drugs, Chinese Herbal (metabolism)
  • Ethanol (toxicity)
  • Hepatocytes (drug effects, pathology)
  • Male
  • Medicine, Chinese Traditional (methods)
  • Metabolome
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Reproducibility of Results
  • Spectrometry, Mass, Electrospray Ionization (methods)
  • Urinalysis (methods)
  • Urinary Tract (drug effects)

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