Comparison of plaque reduction neutralisation test (PRNT) and measles virus-specific IgG ELISA for assessing immunogenicity of measles vaccination.

Abstract

BACKGROUND:

Plaque reduction neutralisation test (PRNT) is a gold standard assay for measles antibodies. PRNT, however, is time-consuming and more difficult to standardise than ELISA.

METHODS:

PRNT results were obtained on pre- and post-vaccination sera from 501 measles-vaccinated infants. From this cohort a sub-set of 210 infants were selected for testing by ELISA, which was performed either manually or by an automated processor. Results were corrected for sampling proportion before comparing the assays.

RESULTS:

Seroconversion was detected by PRNT in 454 infants of whom 324 were estimated to have seroconverted by manual ELISA and 191 by automated ELISA It is recommended that ELISA negative post-vaccination samples are re-tested by PRNT to detect all seroconversions.

• Authors: B J Cohen, D Doblas, N Andrews
• Journal: Vaccine (Vaccine) Vol. 26 Issue 50 Pg. 6392-7 (Nov 25 2008) ISSN: 0264-410X [Print] Netherlands
• PMID: 18834911 (Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Immunoglobulin G
• Measles Vaccine

Topics

• Antibodies, Viral (blood, immunology)
• Antibody Specificity
• Enzyme-Linked Immunosorbent Assay
• Humans
• Immunoglobulin G (blood, immunology)
• Infant
• Measles (immunology, prevention & control)
• Measles Vaccine (administration & dosage, immunology)
• Measles virus (immunology)
• Neutralization Tests
• Predictive Value of Tests
• Sensitivity and Specificity
• Vaccination
• Viral Plaque Assay