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Levels of circulating cell-free nuclear and mitochondrial DNA in benign and malignant ovarian tumors.

AbstractOBJECTIVE:
To analyze the levels of circulating cell-free nuclear DNA and circulating cell-free mitochondrial DNA in patients with benign and malignant ovarian tumors using a gold-standard assay and to investigate whether quantitative alterations of the circulating cell-free species have values in the management of the patients.
METHODS:
One hundred four patients were recruited for this study. We developed a quantitative, multiplex polymerase chain reaction to measure the levels of circulating cell-free nuclear DNA and circulating cell-free mitochondrial DNA in serum and plasma of patients with epithelial ovarian cancer, benign epithelial ovarian tumors, or endometriosis. The levels of the circulating cell-free DNA were compared with those of a healthy, age-matched control group.
RESULTS:
The patients with epithelial ovarian cancer had significantly higher amounts of circulating cell-free nuclear DNA and circulating cell-free mitochondrial DNA in plasma compared with the healthy control group (mean of nuclear DNA 10,723/2,591 and mean of mitochondrial DNA 4,918,978/2,294,264, P=.009 and 0.022, respectively) and with the other group with benign ovarian diseases (mean of nuclear DNA 10,723/2,965 and mean of mitochondrial DNA 4,918,978/1,597,551, P=.027 and 0.002, respectively). However, no relationship between levels of the circulating cell-free DNA and the pathological parameters as well as CA 125 measurement in patients with epithelial ovarian cancer was found. A significant difference between the epithelial ovarian cancer and endometriosis group was found in circulating cell-free mitochondrial DNA but not in circulating cell-free nuclear DNA (mean of mitochondrial DNA 4,918,978/2,273,988 and mean of nuclear DNA 10,723/3,291, P=.013 and 0.105, respectively).
CONCLUSION:
Elevated levels of circulating cell-free nuclear DNA and circulating cell-free mitochondrial DNA in epithelial ovarian cancer may have diagnostic value. Our finding suggests that the circulating molecules might be potential biomarkers in the disease.
AuthorsRebecca R Zachariah, Seraina Schmid, Nicole Buerki, Ramin Radpour, Wolfgang Holzgreve, Xiaoyan Zhong
JournalObstetrics and gynecology (Obstet Gynecol) Vol. 112 Issue 4 Pg. 843-50 (Oct 2008) ISSN: 0029-7844 [Print] United States
PMID18827127 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Biomarkers, Tumor
  • DNA, Mitochondrial
  • DNA, Neoplasm
Topics
  • Adult
  • Biomarkers, Tumor (blood)
  • Cell-Free System (chemistry)
  • DNA, Mitochondrial (blood)
  • DNA, Neoplasm (blood)
  • Endometriosis (blood)
  • Female
  • Humans
  • Ovarian Neoplasms (blood)
  • Polymerase Chain Reaction (methods)
  • ROC Curve

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