Analysis of urinary andrographolides and antioxidant status after oral administration of Andrographis paniculata leaf extract in rats.

Abstract

A simple high-performance liquid chromatography (HPLC) method was developed to determine the content of andrographolide (AP) and 14-deoxy-11,12-dideoxyandrographolide (DIAP) in a pooled urine of rat obtained within 24h after an oral dose of Andrographis paniculata leaf extract at 1g/kg body weight. Cumulative urinary excretion of AP and DIAP in 24h after oral administration of the extract was 0.88% and 1.61% of oral dose administered, respectively. The extract showed significant reduction (p<0.05) of MDA levels and elevation of total antioxidant status in rat urine samples collected in 24 after oral administration.

• Authors: G A Akowuah, I Zhari, A Mariam
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 46 Issue 12 Pg. 3616-20 (Dec 2008) ISSN: 0278-6915 [Print] England
• PMID: 18824206 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Diterpenes
• Plant Extracts
• andrographolide

Topics

• Administration, Oral
• Andrographis (chemistry)
• Animals
• Antioxidants (metabolism)
• Chromatography, High Pressure Liquid
• Diterpenes (urine)
• Lipid Peroxidation (drug effects)
• Plant Extracts (pharmacology)
• Plant Leaves (chemistry)
• Rats
• Rats, Sprague-Dawley
• Reference Standards
• Reproducibility of Results