Immunogenicity and protective efficacy of tuberculosis subunit vaccines expressing PPE44 (Rv2770c).

Abstract

In this study we have evaluated the vaccine potential of a Mycobacterium tuberculosis antigen of the PPE protein family, namely PPE44 (Rv2770c). PPE44-specific immune responses could be detected in mice acutely, chronically and latently infected with M. tuberculosis. Vaccination of mice with a plasmid DNA vaccine coding for PPE44 or recombinant PPE44 protein formulated in adjuvant generated strong cellular and humoral immune responses; immunodominant T cell epitopes were identified. Most importantly, vaccination of mice with both subunit vaccines followed by an intratracheal challenge with M. tuberculosis resulted in a protective efficacy comparable to the one afforded by BCG. Taken together these results indicate that PPE44 of M. tuberculosis is a protective antigen that could be included in novel subunit TB vaccines and that warrants further analysis.

• Authors: Marta Romano, Laura Rindi, Hannelie Korf, Daniela Bonanni, Pierre-Yves Adnet, Fabienne Jurion, Carlo Garzelli, Kris Huygen
• Journal: Vaccine (Vaccine) Vol. 26 Issue 48 Pg. 6053-63 (Nov 11 2008) ISSN: 0264-410X [Print] Netherlands
• PMID: 18822333 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• Antibodies, Bacterial
• Antigens, Bacterial
• BCG Vaccine
• Cytokines
• Epitopes
• PPE44 protein, Mycobacterium tuberculosis
• RNA, Messenger
• Tuberculosis Vaccines
• Vaccines, DNA
• Vaccines, Synthetic
• Virulence Factors
• DNA

Topics

• Adjuvants, Immunologic
• Amino Acid Sequence
• Animals
• Antibodies, Bacterial (biosynthesis)
• Antibody Formation (immunology)
• Antigens, Bacterial (genetics, immunology)
• BCG Vaccine (immunology, therapeutic use)
• Cytokines (biosynthesis)
• DNA (biosynthesis, genetics, immunology)
• Epitopes (genetics, immunology)
• Immunity, Cellular (immunology)
• Immunotherapy, Adoptive
• Mice
• Mice, Inbred Strains
• Molecular Sequence Data
• Mycobacterium tuberculosis (immunology)
• Phylogeny
• RNA, Messenger (biosynthesis, genetics)
• Tuberculosis Vaccines (genetics, immunology)
• Tuberculosis, Pulmonary (immunology, prevention & control)
• Vaccines, DNA (immunology)
• Vaccines, Synthetic (immunology)
• Virulence Factors (immunology)