Rhodamine-loaded poly(lactic-co-glycolic acid) nanoparticles for investigation of in vitro interactions with breast cancer cells.

Abstract

Nanoparticle-based drug delivery systems are considered promising for the delivery of imaging agents and drugs for the detection and treatment of illnesses, including cancer. Investigation of nanoparticle interactions with the diseased cells can lead to better designs. In this work, poly(lactic-co-glycolic acid) nanoparticles loaded with rhodamine 6G were prepared by nanoprecipitation with high encapsulation efficiency. In vitro release studies demonstrated that rhodamine escaped from the nanoparticles at a very slow rate at physiological pH, thus making it ideal for imaging studies. At acidic pH this agent was released quickly, suggesting charge interactions between the polymer and rhodamine. Microscopy and flow cytometry studies show higher uptake in MDA-MB-231 breast cancer cells when exposed to rhodamine-loaded nanoparticles than to rhodamine in solution.

• Authors: Tania Betancourt, Kunal Shah, Lisa Brannon-Peppas
• Journal: Journal of materials science. Materials in medicine (J Mater Sci Mater Med) Vol. 20 Issue 1 Pg. 387-95 (Jan 2009) ISSN: 0957-4530 [Print] United States
• PMID: 18815729 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Biocompatible Materials
• Fluorescent Dyes
• Rhodamines
• Polylactic Acid-Polyglycolic Acid Copolymer
• Polyglycolic Acid
• Lactic Acid

Topics

• Biocompatible Materials (chemistry)
• Biological Transport, Active
• Breast Neoplasms (metabolism, pathology)
• Cell Line, Tumor
• Drug Delivery Systems
• Female
• Flow Cytometry
• Fluorescent Dyes (administration & dosage)
• Humans
• In Vitro Techniques
• Lactic Acid (chemistry)
• Materials Testing
• Microscopy, Confocal
• Microscopy, Electron, Scanning
• Nanoparticles (chemistry, ultrastructure)
• Polyglycolic Acid (chemistry)
• Polylactic Acid-Polyglycolic Acid Copolymer
• Rhodamines (administration & dosage)