Magnesium (Mg) has been proposed to take part in biochemical dysregulation contributing to
psychiatric disorders. The aims of this study was to estimate acute behavioural responses to
clonidine (0.1 mg/kg i.p.),
d-amphetamine (5 mg/kg, i.p),
arecoline (15 mg/kg i.p),
nicotine (6 mg/kg i.p.),
apomorphine (1.5 mg/kg i.p.) and L-5-hydroxytryptophan (300 mg/kg i.p.) in rats fed with Mg-deprivated diet for 49 days and then treated with organic and inorganic Mg
salts (50 mg Mg per kg)
ether alone or in combination with
pyridoxine (5 mg
vitamin B6 per kg). In our study Mg-deficient rats were more sensitive to
d-amphetamine-induced motor stereotypes compared with control rats; time of onset of the stereotypies insignificantly decreased by 14.89% and duration of the stereotypies significantly increased by 19.44% (320.36 +/- 19.90 vs. 268.23 +/- 8.17 minutes; p = 0.043). Mg deficiency did not modulate sensitivity to
nicotine-induced seizure. The time between
nicotine injection and emergence of clonic seizure (seizure latency) in the controls and Mg-deficient rats were 0.80 +/- 0.26 and 0.96 +/- 0.21 minutes respectively. Duration of the
seizures in the controls and Mg-deficient rats were 64.93 +/- 7.20 and 79.32 +/- 8.13 minutes. In our study, Mg deficiency did not affect on
clonidine- and
apomorphine-
induced hypothermia.
Clonidine produced similar decreases in rectal temperature in controls and Mg-deficient group. In experiments using
apomorphine, values of
hypothermia were similar to those observed with
clonidine. Mg deficiency antagonized 5-hydroxytryptophan-induced head-twitch response. The number of head twitches produced by
5-hydroxytryptophan was significantly (p = 0.49) decreased: twofold in
magnesium-deficient rats (1.23 +/- 0.44 per minute) as compared with controls (2.42 +/- 0.52 per minute).
Arecoline-induced
tremor was comparably less expressed in Mg-deficient rats than in controls. The time between
arecoline injection and time of onset of the
tremor in the controls and Mg-deficient rats were 92.75 +/- 19.35 and 245.17 +/- 121.86 seconds respectively (p < or = 0.035). Duration of the
tremors in the controls and Mg-deficient rats were 1175.58 +/- 127.87 and 703.83 +/- 89.33 seconds (p = 0.015).
Magnesium salts (Mg
chloride, Mg
L-aspartate alone and in combination with B6) were administered through gastric tube during twenty days up to complete compensation oferythrocyte and plasma Mg levels in all experimental groups. In our study administration of Mg
salts resulted in normalization of acute behavioural responses in Mg-deficient rats to
d-amphetamine,
arecoline, and L-5-hydroxytryptophan. Behavioural responses in rats treated with both Mg
chloride and Mg
L-aspartate in combinations with B6 were comparable with those observed in MagneB6 treatment.