HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Mechanisms of ileal adaptation for glucose absorption after proximal-based small bowel resection.

AbstractINTRODUCTION:
The hexose transmembrane transporters SGLT1 and GLUT2 are present in low quantities in ileum where little glucose absorption occurs normally; however, glucose uptake in ileum is highly adaptable after small bowel resection.
HYPOTHESIS:
Ileal adaptability for glucose absorption after jejunal resection is mediated predominately by upregulation of GLUT2.
METHODS:
Rats underwent 70% proximal-based jejunoileal resection. Transporter-mediated glucose uptake was measured in proximal and distal remnant ileum 1 and 4 wk postoperatively (n = 6 rats, each) and in corresponding ileal segments in control and 1 wk sham laparotomy rats (n = 6, each) without and with selective inhibitors of SGLT1 and GLUT2. In separate groups of rats (n = 6, each), protein (Western blots), mRNA (reverse transcriptase polymerase chain reaction [RT-PCR]), and villus height (histomorphology) were measured.
RESULTS:
After 70% proximal intestinal resection, there was no dramatic change in protein or mRNA expression per cell of either SGLT1 or GLUT2, but median glucose uptake (nmol/cm/min) increased markedly from 52 (range 28-63) in controls to 118 (range 80-171) at 1 wk, and 203 (range 93-248) at 4 wk (p < or = 0.04 each) correlating with change in villus height (p < or = 0.03).
CONCLUSIONS:
Ileal adaptation for glucose transport occurs through cellular proliferation (hyperplasia) and not through cellular upregulation of glucose transporters.
AuthorsC W Iqbal, H G Qandeel, Y Zheng, J A Duenes, M G Sarr
JournalJournal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract (J Gastrointest Surg) Vol. 12 Issue 11 Pg. 1854-64; discussion 1864-5 (Nov 2008) ISSN: 1873-4626 [Electronic] United States
PMID18766411 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References
  • Glucose Transporter Type 2
  • RNA, Messenger
  • Sodium-Glucose Transporter 1
  • Glucose
Topics
  • Adaptation, Physiological
  • Animals
  • Disease Models, Animal
  • Glucose (metabolism)
  • Glucose Transporter Type 2 (metabolism)
  • Intestinal Absorption (physiology)
  • Jejunum (metabolism, surgery)
  • Male
  • Probability
  • RNA, Messenger (analysis)
  • Random Allocation
  • Rats
  • Rats, Inbred Lew
  • Reference Values
  • Sensitivity and Specificity
  • Short Bowel Syndrome (metabolism)
  • Sodium-Glucose Transporter 1 (metabolism)
  • Statistics, Nonparametric

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: