Abstract | INTRODUCTION: The hexose transmembrane transporters SGLT1 and GLUT2 are present in low quantities in ileum where little glucose absorption occurs normally; however, glucose uptake in ileum is highly adaptable after small bowel resection. HYPOTHESIS: Ileal adaptability for glucose absorption after jejunal resection is mediated predominately by upregulation of GLUT2. METHODS: Rats underwent 70% proximal-based jejunoileal resection. Transporter-mediated glucose uptake was measured in proximal and distal remnant ileum 1 and 4 wk postoperatively (n = 6 rats, each) and in corresponding ileal segments in control and 1 wk sham laparotomy rats (n = 6, each) without and with selective inhibitors of SGLT1 and GLUT2. In separate groups of rats (n = 6, each), protein (Western blots), mRNA ( reverse transcriptase polymerase chain reaction [RT-PCR]), and villus height (histomorphology) were measured. RESULTS: After 70% proximal intestinal resection, there was no dramatic change in protein or mRNA expression per cell of either SGLT1 or GLUT2, but median glucose uptake (nmol/cm/min) increased markedly from 52 (range 28-63) in controls to 118 (range 80-171) at 1 wk, and 203 (range 93-248) at 4 wk (p < or = 0.04 each) correlating with change in villus height (p < or = 0.03). CONCLUSIONS: Ileal adaptation for glucose transport occurs through cellular proliferation ( hyperplasia) and not through cellular upregulation of glucose transporters.
|
Authors | C W Iqbal, H G Qandeel, Y Zheng, J A Duenes, M G Sarr |
Journal | Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract
(J Gastrointest Surg)
Vol. 12
Issue 11
Pg. 1854-64; discussion 1864-5
(Nov 2008)
ISSN: 1873-4626 [Electronic] United States |
PMID | 18766411
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
|
Chemical References |
- Glucose Transporter Type 2
- RNA, Messenger
- Sodium-Glucose Transporter 1
- Glucose
|
Topics |
- Adaptation, Physiological
- Animals
- Disease Models, Animal
- Glucose
(metabolism)
- Glucose Transporter Type 2
(metabolism)
- Intestinal Absorption
(physiology)
- Jejunum
(metabolism, surgery)
- Male
- Probability
- RNA, Messenger
(analysis)
- Random Allocation
- Rats
- Rats, Inbred Lew
- Reference Values
- Sensitivity and Specificity
- Short Bowel Syndrome
(metabolism)
- Sodium-Glucose Transporter 1
(metabolism)
- Statistics, Nonparametric
|