Abstract |
Our earlier studies identified a fraction (F2) of Leishmania donovani soluble promastigote antigen belonging to 97.4-68 kDa for its ability to stimulate Th1-type cellular responses in cured visceral leishmaniasis (VL) patients as well as in cured hamsters. A further fractionation of F2-fraction into seven subfractions (F2.1-F2.7) and re-assessment for their immunostimulatory responses revealed that out of these, only four (F2.4-F2.7) belonging to 89.9-97.1 kDa, stimulated remarkable Th1-type cellular responses either individually or in a pooled form (P4-7). In this study these potential subfractions were further assessed for their prophylactic potential in combination with BCG against L. donovani challenge in hamsters. Optimum parasite inhibition ( approximately 99%) was obtained in hamsters vaccinated with pooled subfractions and they survived for 1 year. The protection was further supported by remarkable lymphoproliferative, IFN-gamma and IL-12 responses along with profound delayed type hypersensitivity and increased levels of Leishmania-specific IgG2 antibody as observed on days 45, 90 and 120 post-challenge suggesting that a successful subunit vaccine against VL may require multiple Th1-immunostimulatory proteins. MALDI-TOF-MS/MS analysis of these subfractions further revealed that of the 19 identified immunostimulatory proteins, Elongation factor-2, p45, Heat shock protein-70/83, Aldolase, Enolase, Triosephosphate isomerase, Disulfideisomerase and Calreticulin were the major ones in these subfractions.
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Authors | Shraddha Kumari, Mukesh Samant, Pragya Misra, Prashant Khare, Brijesh Sisodia, Ajit K Shasany, Anuradha Dube |
Journal | Vaccine
(Vaccine)
Vol. 26
Issue 45
Pg. 5700-11
(Oct 23 2008)
ISSN: 0264-410X [Print] Netherlands |
PMID | 18762224
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Protozoan
- Cytokines
- Leishmaniasis Vaccines
- Polyproteins
- Protozoan Proteins
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Topics |
- Animals
- Antibodies, Protozoan
(blood)
- Cricetinae
- Cytokines
(metabolism)
- Hypersensitivity, Delayed
- Leishmania donovani
(growth & development, immunology, metabolism)
- Leishmaniasis Vaccines
(administration & dosage, immunology)
- Leishmaniasis, Visceral
(immunology, parasitology, prevention & control)
- Lymphocyte Activation
- Male
- Mesocricetus
- Polyproteins
(immunology)
- Protozoan Proteins
(immunology)
- Solubility
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Th1 Cells
(immunology)
- Vaccination
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