Protective role of withaferin-A on 7,12-dimethylbenz(a)anthracene-induced genotoxicity in bone marrow of Syrian golden hamsters.

Abstract

The present study has investigated the antigenotoxic effect of withaferin-A, a steroidal lactone obtained from the roots and leaves of Withania somnifera, in 7,12-dimethylbenz(a)anthracene (DMBA)-induced genotoxicity. Measurement of the frequency of micronucleated polychromatic erythrocytes (MnPCEs) and chromosomal aberrations is used as cytogenetic endpoints. A single intraperitoneal injection of DMBA (30 mg/kg b.w.) to golden Syrian hamsters resulted in marked elevation in the frequency of MnPCEs and aberrations in the chromosomal structure. Hamsters pretreated with withaferin-A intraperitonealy 2 h before the injection of DMBA, significantly reduced the frequency of MnPCEs and chromosomal aberrations such as chromosomal break, gap, minute, and fragment. Our results thus demonstrated the antigenotoxic effect of withaferin-A in DMBA-induced genotoxicity in the bone marrow of golden Syrian hamsters.

• Authors: K Panjamurthy, S Manoharan, Venugopal P Menon, Madhavan R Nirmal, N Senthil
• Journal: Journal of biochemical and molecular toxicology (J Biochem Mol Toxicol) 2008 Jul-Aug Vol. 22 Issue 4 Pg. 251-8 ISSN: 1099-0461 [Electronic] United States
• PMID: 18752311 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antimutagenic Agents
• Mutagens
• Withanolides
• 9,10-Dimethyl-1,2-benzanthracene
• withaferin A
• Ergosterol

Topics

• 9,10-Dimethyl-1,2-benzanthracene (antagonists & inhibitors, toxicity)
• Animals
• Antimutagenic Agents (isolation & purification, pharmacology)
• Bone Marrow (drug effects)
• Chromosome Aberrations (drug effects)
• Cricetinae
• Ergosterol (analogs & derivatives, isolation & purification, pharmacology)
• Erythrocytes (drug effects)
• Male
• Medicine, Ayurvedic
• Mesocricetus
• Micronuclei, Chromosome-Defective (drug effects)
• Micronucleus Tests
• Mutagens (toxicity)
• Withania (chemistry)
• Withanolides