Abstract |
Although diesel exhaust particles ( DEP) exacerbate murine allergic asthma, the mechanisms remain unclarified. This study elucidated the effects of pulmonary exposure to DEP on extrathoracic allergen-induced CD4 polarization using an ex vivo system in asthmatic mice. Mice were divided into 4 groups, and vehicle, DEP, ovalbumin (OVA), or DEP + OVA was intratracheally instilled biweekly 5 times. Twenty-four h after the last intratracheal instillation, the spleen was removed. Mononuclear cells were isolated from the spleen and stimulated in vitro with OVA; thereafter, the number of separated CD4(+) cells was counted. The number of separated CD4(+) cells was significantly greater in the OVA or the DEP + OVA group than in the vehicle group. Furthermore, the number was significantly greater in the DEP + OVA than in the DEP or the OVA group. Taken together, in vivo DEP exposure amplified extrathoracic CD4 polarization in asthmatic subjects.
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Authors | Ken-Ichiro Inoue, Eiko Koike, Rie Yanagisawa, Hirohisa Takano |
Journal | Immunopharmacology and immunotoxicology
(Immunopharmacol Immunotoxicol)
Vol. 31
Issue 1
Pg. 71-4
( 2009)
ISSN: 1532-2513 [Electronic] England |
PMID | 18752167
(Publication Type: Journal Article)
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Chemical References |
- Vehicle Emissions
- Ovalbumin
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Topics |
- Animals
- Asthma
(chemically induced, immunology)
- CD4-Positive T-Lymphocytes
(cytology, drug effects, immunology)
- Cell Count
- Lymphocyte Activation
(immunology)
- Male
- Mice
- Mice, Inbred ICR
- Ovalbumin
(administration & dosage, immunology)
- Spleen
(cytology, drug effects, immunology)
- Vehicle Emissions
(toxicity)
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